What are transposons? How are they indentified in a genome? What is the mechanism of transposition?
Bacterial mRNA AUGUUUGCUGGGGGACAUUCGUGGGCA produced the following template strand from which this mRNA was transcribed TACAAACCACCCCCTGTAAGCACCCGT My questiohn is inserted a T at the begining of the sequence to produce TTACAAACGACCCCCTGTAAGCACCCGT what is the new mRNA sequence corresponding with this altered DNA Deletetion of th
In Aspergillus, the genes y (yellow), os (osmotic), c (colonial), leu (leucine-requiring), and a (aconidial) are located on the long arm of chromosome 3. A diploid was made between a haploid wild type and a haploid of genotype y os c leu a. From the green diploid, yellow diploid sectors appeared. When these were sampled and test
How can only four different nucleotides be used to construct a genetic code that will specify 20 different amino acids? These questions are presented.
What is a-complementation and what are some examples of this process?
The Polymerase Chain Reaction (PCR), devised by Kary Mullis in the mid-1980's has revolutionized molecular biology by allowing a whole new approach to the study and analysis of genes. i) What is PCR? ii) What are the steps involved? iii) What are the necessary reagents involved in making the PCR work?
From the sequence below, find probable restriction endonuclease sites. HINT: The recognition sequences are 6bp long. 5' CATTCGGTACCTTCGTGAATTCTGACCAC 3'
What are the key differences between DNA and RNA in both structure and function (without taking supercoiling into consideration)?
If the adenine content of DNA from an organism is 36%, what is the guanine content?
Define Real Time PCR
In sheep the polled condition ( hornless ) is controlled by a single gene locus with two alleles. Heterozygous females are polled but heterozygous males have horns. Suppose two Heterozygous sheep were crossed. Refer to attachment for multiple choice questions.
Analysis of base composition from DNA extraction in Aspergillus niger was seen to contain 37% cytosine. Using this data predict the % of adenine. If the same were detected in Tobacco Mosaic Virus [TMV], will it be possible to predict the % of adenine. This job gives reasons.
A double stranded DNA virus has 200,000 base pairs. Determine the number of nucleotides present and the total number of complete spirals. How long will this DNA be?
Molecular weight of DNA in an autosome of human beings is 0.5 à? 10 power 12. Determine the number of deoxyribonucleotide pairs in this DNA and calculate the length in both micrometers and inches.
A solution containing 80% cytosine and 20% uracil was used to make synthetic RNAs. A bacterial cell free system produced proteins using these mRNAs in the following proportions: a) 4 times as many serine as leucine residues. b) 16 times as many serine residues as phenylalanine residues. c) 16 times as many proline resid
Convert the following mRNA segments into their polypeptide equivalents; a) 5′ GAAAUGGCAGUUUAC 3′ b) 3′ UUUUCGAGAUGUCAA 5′ c) 5′ AAAACCUAGAACCCA 3′ d) 3′ UGACCAGAUCUACCGAAA 5′
A certain gene consists of 450 nucleotide pairs. The protein product of the gene consists of 150 amino acid residues. How many codons make the 150 residues?
How many different mRNAs would specify the following amino acid sequences - a) met - phe - ser - pro b) ile - arg - phe - leu - ala [use the triplet codon chart to find the number of codons representing the amino acids]
Suppose 25,000 μm is 1 inch. Enumerate a formula to find the length of a DNA molecule in inches a) if the length is known in μm and b) only the number of nucleotide pairs is given.
DNA of a certain species of yeast has thymidilic acid content of 32.6%. If the A/T ratio is 0.97, what is the composition of deoxyadenylic acid?
In one set of Φ X phages the deoxyribonucleotide composition was A = 24.7, T = 32.7, G = 24.1 and C = 18.5. Another set of phages had the following deoxyribonucleotide composition - A = 27.6, T = 27.8, G = 22.3 and C = 22.3. a) Find the A/T, G/C ratio of the 2nd set of phages. b) What is the structure of the phage DNA wit
In a mutation experiment in drosophila, 45 mutations were detected amongst 543 males that received 2500 Roentgens (R) and 67 mutations in 649 males that received 4000R. What is the number of progeny expected in 1000 progeny of males exposed to 6000R?
Under laboratory conditions, a synthetic polyribonucleiotide was produced using a mixture of A and G with a relative frequency of 5:1 respectively. How would a scientist predict the relative frequencies in which the triplets are expected to form when the ribonucleotides form in a random linear array? Indicate which amino acid(s)
Find the number of triplet codons feasible from 4 ribonucleotides A, U, G and C containing a) no uracil, b) one or more uracils.
An animal cell has 46 post-mitotic chromosomes in each of its somatic cells. How many chromosomes should be present in the following cells- a) Mature egg, b) 1st polar body, c) Sperm, d) Spermatid, e) Primary spermatocyte, f) Brain cell, g) Secondary oocyte, h) Spermatogonium.
Cross between pure red tomato and yellow tomato produced all red F1 progeny. From the F2 progeny of 400 plants, 90 were yellow. Hypothesize that a single pair of allele is involved such that Y- = Red and yy = Yellow. Test this hypothesis using Chi square test.
In squash, disc shaped fruit is dependent on dominant gene D and elongated fruit on recessive allele d. Yellow color is controlled by dominant gene R and green color by recessive allele r. Draw a cross between the homozygous squash fruits and summarize the expected results of F2.
Garden pea producing round seeds were crossed with those producing wrinkled seeds. The result was the production of 5474 round seeds and 1850 wrinkled seeds. a) What is the original parental cross? b) Indicate the gamete types c) F1 progeny? d) What result do you expect when F1 is selfed? e) Indicate F1 selfed ga
Predict the results of the following crosses: a) Tall [dominant and homozygous] with dwarf - F1? b) Product of selfed F1. c) F1 with original tall parent. d) F1 with original dwarf parent
Avena stirigosa [oats] is a diploid species with 2n=14 chromosomes. How many different types of gametic combinations can form in this species?