Explore BrainMass
Share

Explore BrainMass

    Genetic Biology Questions

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    Genetic Biology Questions. See attached file for full problem description.

    © BrainMass Inc. brainmass.com October 9, 2019, 7:05 pm ad1c9bdddf
    https://brainmass.com/biology/dna-chromosomes-and-genomes/genetic-biology-questions-107487

    Attachments

    Solution Preview

    1. To solve this restriction map, you have to make a few assumptions and see if it holds true. Notice that the 2.5 Kb fragment of the SmaI digest still exists in the double digest. In addition, the 3.5 Kb XbaI fragment exists in the double digest. Therefore, we know that these two fragments are at the ends. Armed with this information, all we need to do is try a few trial and error fragments for the remaining restriction sites to see how it all fits together. Here's the final map:

    3.5 Kb --- XbaI --- 0.5 Kb --- SmaI --- 6.0 Kb --- XbaI --- 1.5 Kb --- SmaI --- 2.5 Kb

    __________________________________________

    2a. Since the ATG is on the 3' side of the first mutation, that mutation wouldn't affect the amino acid sequence of the polypeptide. Exchanging one purine for another purine (or one pyrimdine for another pyrimidine) is a transition. Therefore, this is a transition. The codons are:

    5' -- ATG CTG GGC ATT CAA CCG

    The amino acid sequence would be: met-leu-gly-ile-gln-pro

    2b. If we remove the T, we get a frameshift mutation that throws everything off 3' of the deletion. The sequence would be:

    5' -- ATG CTG GGC ATC AAC ...

    The amino acid ...

    $2.19