Specificity of Albumin Binding
Not what you're looking for?
In this experiment we used electrophoresis to study the binding interactions that occure between serum albumin and two synthetic dyes. The dyes were Bromophenol Blue and Ponceau S. The basis for this procedure was the observation that the free dyes not bound to albumin migrate faster than albumin or dyes bound to albumin.
Important: In the electrophoresis gel there are two wells that have the number 4 tube because someone in our group accidentally filled up two of the wells with tube #4 mixture. If you have any questions please contact me.
See attached file for full problem description.
Purchase this Solution
Solution Preview
Here's a great title for the experiment: Determining the Relative Affinities of Two Dyes, Bromophenol Blue and Ponceau S, to Bovine Serum Albumin using Electrophoresis
Explanation of the experiment:
Lane 1: We see the migration of bromophenol blue when not bound to BSA (bovine serum albumin). It migrates fairly quickly through the gel.
Lane 2: When we add BSA to the dye, we notice that the location of the dye is farther up the gel. That means that the dye has not migrated as quickly. Its movement through the gel matrix has been impeded. How come? It has become bound to BSA. This new complex (BSA-bromophenol blue) migrates slower through the gel than does bromophenol blue on its own. This demonstates a nice binding affinity between BSA and bromophenol.
Lane 3: This is much the same as Lane 2 except for the fact that we can just begin to see some excess unbound bromophenol blue appearing in the zone where free bromophenol blue migrates.
Lane 4: We'll ignore the first lane 4 and look only at the second lane 4. This clearly shows that the more ...
Purchase this Solution
Free BrainMass Quizzes
Basic Concepts in Neuroscience
This quiz provides a review of the basic concepts in neuroscience.
How Much Do You Know About Genetic Inheritance?
Most of us studied the basics of dominant and recessive genes at some point. However, genetics are much more complicated than this. How far beyond the basics does your knowledge go?
Breastfeeding Basics
How much do you know about breastfeeding? Find out with this quiz!
Comfort Measures For Labor
Are you ready to doula someone through labor?
Vision and Oculomotor Control
This quiz will test the student's knowledge of the neural underpinnings of the visual system and its central pathways.