A) What are some of the unique circumstances of forensic genetic evidence? B) Describe some of the limitations involved with processing forensic DNA samples?
Forensic Science - Based on this tree of mtDNA inheritance, give the haplotypes for individuals 2, 3, and 14.
I've attached the assignment so please download the file. Thanks!
A strain of Aspergillus was subjected to mutagenesis by X rays, and two tryptophan-requiring mutants ( A and B) were isolated. These tryptophan-requiring strains were plated in large numbers to obtain revertants to wild type. You failed to recover any revertants from mutants A and recovered one revertant from mutant B. This r
Please see attachment for image. You ran your experiments on a gel and detected the proteins using a Western Blot (again remind yourself how do you make recombinant proteins, generate antibodies, and perform a Western). You results are demonstrated on this gel. The lanes are designated with the letter A-G. Vertical lines are
3. Explain why teratogens are difficult to identify. 4. Explain why a woman carrying the gene for hemophilia can produce two hemophiliac sons when she is mated to a normal male. 5. Under what conditions does a female acquire an X-linked recessive disorder? 7. The pedigree for Queen Victoria of England, a carrier
An allele that has no noticeable effect on the appearance of an organism unless it is found in the homozygous condition is
1. With incomplete inheritance a. Traits show either dominance or recessiveness b. Heterozygotes are phenotypic intermediates c. Phenotypic traits have a continuous nature d. Genetic counseling is useful 2. An allele that has no noticeable effect on the appearance of an organism unless it is found in the homo
Please see attached file. 1. A stable polyploidy plant species was found that has 26 chromosomes, which form 13 bivalents in meiosis. Is it an autoploid or alloploid? 2. Horses have 64 chromosomes, while donkeys have 62. Hybrids between horses and donkeys are mules, and are sterile. If two mules mated, with each prod
Please answer in detail. 3. Oats are allohexaploid with 2N=6X=42. How many chromosomes does one of its diploid relatives, Avena barbata, have? 4. Arabidopsis thaliana has 5 pairs of chromosomes, so 2n=2x=10. Answer the following questions about this plant. a. How many chromosomes would an autotetraploid
In fruit flies, black body (b) is recessive to gray body (b+), purple eyes (pr) is recessive to red eyes (pr+), and vestigial wings (vg) are recessive to normal wings (vg+). The loci coding for these traits are linked, with the following map distances: b____5_____pr_______5_______vg The interference among these gen
The common fruit fly, Drosophila melanogaster, has been the subject of genetic study for over 100 years. In fruit flies, curly wings are dominant to straight wings. 1. How would you designate the genotype of a homozygous curly-winged fly? _________ 2. How would you designate the genotype of a straight-winged fly? ________
In the following sequence find restriction sites for EcoRI, HindIII and Hae III. Show how many fragments will be produced by restriction by all of these enzymes at the same time and their size, Which of the fragments produced will have 5'-overhang, 3'-overhang or blunt end? GAAGAACCTGAATTCAAATTTGGCCCTGCTGCTGAAGCTTGCTGACCAGG
Suppose you were to construct a synthetic RNA from a repeating dinucleotide AGAGAGAGAG and then use it as a messenger to synthesize a polypeptide in an invito system. What type of polypeptide would you make from this polynucleotide? Would you expect to have more than one polypeptide? Why?
The human beta-globin polypeptide is 146 amino acids long. How long is the coding portion of the human beta-globin mRNA?
1. What sequence of nucleotide pairs in a Drosophila gene will encode the amino acid sequence met-trp-phe-trp-met (reading from the amino terminus to the carboxyl terminus)? 2. A wild type gene contains the trinucleotide-pair sequence: was 5'-GAG-3' changed to:5'-GTG-3' 3'CTC -5' 3'-CAC-5' If the second base
I need to complete a lab can you tell me what I will see: 1. Describe the appearance of the DNA as it precipitates out of solution. 2. Describe the appearance of the DNA after you have spooled it. 3. Describe the appearance of the DNA after you let it dry.
Although normal E.coli cells can synthesize all the amino acids, some mutants called amino acid auxotrophs, are unable to synthesize specific amino acids and require the addition of that amino acid to the culture medium for optimal growth. In addition to their role in protein synthesis, specific amino acids are also required in
Affinity Chromatography. Please follow directions and problems attached in Word Doc 9. Word Doc 2 explains the procedure used. See attached file for full problem description.
Describe what a "gene" is. Include a definition in addition to information on the structure of a gene & regulatory regions.
How many DNA fragments would be generated and what would be the size of each fragment if the restriction enzyme HaeIII was mixed with the following DNA sequence? 5' ACCGGCATTACGGCCTTACATGGCCATAGCCGGAACATCGT 3' 3' TGGCCGTAATGCCGGAATGTACCGGTATCGGCCTTGTAGCA 5'
What are the physical changes needed in the body that would need to happen in order for our bodies to live forever. Changes such as telomerase activity, collagen flexibility, environmental changes, role of the immune system, DNA repair enzymes: these all come into play. Construct a brief list (not to exceed 100 carefully
Purine biosynthesis is allowed to take place in the presence of [N15]aspartate, and the newly synthesized GTP and ATP are isolated. What positions are labeled in the two nucleotides?
Having a problem understanding Mendel's theory, would like help answering these scenario's. Have to write an report based on these questions please help Assume that "S" represents the dominant trait of having long leaves, and "s" represents the recessive short-leafed trait in a plant. In the parental generation, you cross
What is the significance of adenine, thymine, guanine, and cytosine in the structure of a DNA molecule? Explain how these four nucleotides bond with each other and other components of DNA. What evidence is there to support that this code carries hereditary instructions? Would like your answers to these questions and
In this experiment we used electrophoresis to study the binding interactions that occure between serum albumin and two synthetic dyes. The dyes were Bromophenol Blue and Ponceau S. The basis for this procedure was the observation that the free dyes not bound to albumin migrate faster than albumin or dyes bound to albumin. I
I do not understand the process of gene expression as given on certain cites. Please explain the process of gene expression in prokaryotes.
3. The urine of some people has a distinctive odor after eating asparagus. This appears to be a recessive trait. If a person with aromatic urine marries a person heterozygous for this trait, what phenotypic and genotypic ratios will their children theoretically exhibit? 4. Cat's tail has 42 chromosomes in each somatic cell.
What is involved in going from genomic DNA to messenger RNA (mRNA)? The transciption process involves the concerted action of several proteins called transcription factors which process the DNA in a multi-step process into mRNA.
Genetic Biology Questions. See attached file for full problem description.
Question 2: Motif finding Try a number of motif-finding algorithms covered in the lab, and try different parameters. What good motif can you find from the sequence? Here are some useful hints for the analysis (depends on the software you are using): ? Motif width ~ 13, and search in both strands of the DNA ? Ask each algori
Study questions and electrophoresis gel are attached.