Agarose gel electrophoresis - why does my bp not add up?
Will be as brief as I can! - any help would be appreciated. used 3 lanes on my gel and ran in each: lane 1: hyperladder (linear fragments) lane 2: EcoR1 digested recombinant pUC18 (size of insert unknown) lane 3: undigested recombinant pUC18 plasmid Results obtained from gel and standard curve: lane 2: 2 bands app