Hi. I have to write a practical report for Glucose Tolerance test. I have tried and tried but am struggling. I need to take into account any sampling and or dilution involved in the experiment and work out a conversion factor(hope that makes sense)so that my results can be expressed as mg of glucose per 100cm3 of original blood sample. I am sure its quite simple but not to me!!!
I think I need to convert the glucose solution (50ug/cm3) to mmol/l. That is also difficult for me. I am not asking for answers just an explanation on how to do it please!
I have included my two attachments (table with absorbance results) and the instructions for the experiment.© BrainMass Inc. brainmass.com October 9, 2019, 8:32 pm ad1c9bdddf
The first thing we need to do is look at how the blood sample was prepared. Keep in mind that 1 cm^3 = 1 ml.
You were told that 0.1 ml of blood was pipetted into 0.9 ml of water in a centrifuge tube. This is a 1:10 dilution. 0.1 ml blood ends up in 1.0 ml solution.
Then, they added 0.5 ml of 5% Zn SO4 and 0.5 ml of 0.3M Ba (OH)2. So, another 1.0 ml of solutions were added, bringing the final solution volume to 2.0 ml.
Therefore, the initial blood sample was diluted 1:20. That is, they took 0.1 ml blood and put it into 2.0 ml of final volume. Whatever we work out as concentration in the blood sample tested in the spectrophotometer, we know that the blood concentration will be 20 times that concentration.
Then, what happened? You took 0.5 ml of ...