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Biological Chemistry

Finding the Amount of Phenol Red in Micrograms

Calculation of amount of phenol red in micrograms present in 3ml of the solution, you will need to use the following data: the concentration of the unknown 5.0 micromoles, volume = 3ml, MW of phenol red = 376.4g.

Net charge on peptide

Consider this peptide: Phe-Glu-Ser-Met What is the net charge of this peptide at ph=1 What is the net charge of this peptide at ph=7

Agarose gel electrophoresis

When digested DNA is analysed using agarose gel electrophoresis the total size of fragments sometimes does not add up to the size of the original DNA molecule. How can this be explained and how can this be seen in ethidium bromide stained agarose gels?

Enzyme and Endocrine Disruptor Clarifications

Which of these apply to Enzymes A. The majority of chemical reactions in our bodies do not require enzymes. B. Enzymes are functional proteins C. Enzymes get used up as part of a reaction D. Cyto chrome P-450 is an example of an enzyme that helps to oxidize chemicals in the body. E. Biotransformation of toxins could not o

Mutagens

A friend insists that all mutagens are dangerous. How would you respond?

Investigation of Fermentation of Yeast: Determination of Glucose Uptake

I carried out the practical shown in the attachment, and am struggling with the calculations, Can anyone please explain what I need to do? As there are different calculations for different bits I am submitting separate post for each bit. The model answers from the experiment are also attached (not my results but I am not ask

Distinctive Parts of a Wheat Kernel

The three distinctive parts of the wheat kernel are the a. germ, the endosperm and the bran b. bran, the flour and the endosperm c. flour, the bran and the germ d. floor, the bran and the starch

Determining Km and Vmax

I have made a lineweaver-burk graph with some data, but I don't know how to determine Vmax and Km from it? Would you please give me an example of how to do it. Please make a lineweaver-graph with some values and tell me how to determine Km and Vmax. I can figure out the slope from the graph, but cannot figure out Y and x in Y

This amino acid displays a free amino group in the peptide ALA-GLN-ARG-SER-HIS

Dear OTA, I have 2 multiple questions as attached. I have done those but have some doubts. The questions are in red and my answer is in black. Need to get explanation why the option B in #61 is wrong (heavy references) and why option A in #53 is wrong (also references) Basically I need to know why only 1 option is good for

Biochemistry and Molecular Characteristics

Question #1: (1 point) In the urea cycle, the molecule that is synthesized in the cytosol and transported to the mitochondrial matrix for subsequent reactions is a.) citrulline b.) ornithine c.) argininosuccinate d.) aspartate e.) carbamoyl-phosphate Question #2: (1 point) Inosine 5'-phosphate is a precursor to which

Calculation of Fractional Saturation of Protein in GDP / GTP

Guanyl nucleotide regulatory proteins (N) alter cell function based on the type of guanyl nucleotide occupying the nucleotide site on the protein. The affinity of this site for guanyl nucleotides is to a certain extent under hormonal control. Given a Ka of the site for guanyl nucleotides of 3 x 10^7 M in the presence of hormon

Beta-Oxidation of Odd and Even Carbon Fatty Acids

1 BCH-100 Fall 2007 BLH #13 (4 points) Question #1: (0.8 point) How many acetyl CoA's will come from a 12 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #2: (0.8 point) How many acetyl CoA's will come from a 13 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #3: (0.8 point) How many p

Fumarase Deficiency

Fumarase deficiency is an extremely rare disease in humans. People with this disease can survive although they are significantly affected. a) Given the importance of fumarase to the citric acid cycle how is it possible for people with fumarase deficiency to produce adequate ATP for survival? b) what would be the overall

Equilibrium Concentration from Keq and Initial Concentration

Part 1: what is equilibrium concentrations and concentration ratios of glucose-6-phosphate/glucose-1-phosphate in the phosphoglucomutase reaction when the initial concentration of glucose-6-phosphate is (a) .01 M and (b) 10^-3 M. The Keq for the reaction below is 19. Glucose-1-phosphate ---> glucose-6-phosphate Part 2: wh

Free Energy of Fructose-6-Phosphate and Glucose-6-Phosphate

Fructose-1,6-diphosphate can be converted to glucose-1-phosphate by three successive reactions: Fructose 1,6 diphosphate + H20 ---> fructose-6-phosphate + Pi Keq= 607 Fructose-6-phosphate ---> glucose-6-phosphate G°= -410 cal/mol Glucose-6-phosphate --->glucose-1-phosphate G°= 1750 cal/mol As

Acid/Base

You want to make a 0.1M acetate buffer from 0.1M solutions of acetic acid and acetate. How many mL of acetic acid would you need per liter of solution (with the rest of the volume coming from the acetate solution) to generate a pH of 4.75? The pKa of acetic acid is 4.76

Pka

The pKA of histidine in a protein active site where it is next to an aspartate or glutamate would be____________ the pKa of free histidine solution higher, lower, no different Is it higher??

Hydrolysis of Phosphocreatine

For the hydrolysis of phosphocreatine: Phosphocreatine + H2O==creatine + Pi the standard state deltaG is -42.8 kJ/mol at 37 degrees celsius. If in a given cell the concentration of creatine is 1.7mM, phosphate is 5.8mM, and phosphocreatine is 6.4mM, what is the deltaG for hydrolysis of phosphocreatine in the cell? Nearest 0.

Delta G

The equilibrium constant for the denaturation of a protein is determined to be 0.13 at 59.8 degrees Celsius. What is the standard state of delta G for the reaction, in kJ/mol to 0.01kJ/mol.

Phosphate System and Physiological pH

The phosphate system serves to buffer the intracellular fluid of cells at physiological pH because pK2 (7.20) lies near the normal pH range of 6.9-7.4. If the total cellular concentration of phosphate is 19mM and the pH is 6.9, what is the concentration of (H2PO4-) in mM?? Is this correct??? H2PO4-(aq) H+(aq) + HPO42-(aq)

Free energy and its affects

What affect does the presence of metal ions like Mg++ in the cell have on free energy of hydrolysis of ATP?? raise, lower, or no affect why??

Glycolysis and Gluconeogenesis

The following questions refer to the mitochondrial enzyme MALATE DEHYDROGENASE. What is the chemical reaction, within the citric acid cycle, that is catalyzed by malate dehydrogenase, and which substrates, products, and/or cofactors are involved? The reaction from the previous question is classified as a dehydrogenatio

Reaction process

Lactate dehydrogenase catalyzes the reversible reaction: Pyruvate + NADH + H^(+) --> Lactate + NAD^(+) Given the following facts, (a) tell in which direction the reaction will tend to go if NAD^(+), NADH, pyruvate, and lactate were mixed, all at 1 M concentrations, in the presence of lactate dehydrogenase at pH 7; (b) calc