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Biological Chemistry

Determining Km and Vmax

I have made a lineweaver-burk graph with some data, but I don't know how to determine Vmax and Km from it? Would you please give me an example of how to do it. Please make a lineweaver-graph with some values and tell me how to determine Km and Vmax. I can figure out the slope from the graph, but cannot figure out Y and x in Y

This amino acid displays a free amino group in the peptide ALA-GLN-ARG-SER-HIS

Dear OTA, I have 2 multiple questions as attached. I have done those but have some doubts. The questions are in red and my answer is in black. Need to get explanation why the option B in #61 is wrong (heavy references) and why option A in #53 is wrong (also references) Basically I need to know why only 1 option is good for

Biochemistry and Molecular Characteristics

Question #1: (1 point) In the urea cycle, the molecule that is synthesized in the cytosol and transported to the mitochondrial matrix for subsequent reactions is a.) citrulline b.) ornithine c.) argininosuccinate d.) aspartate e.) carbamoyl-phosphate Question #2: (1 point) Inosine 5'-phosphate is a precursor to which

Calculation of Fractional Saturation of Protein in GDP / GTP

Guanyl nucleotide regulatory proteins (N) alter cell function based on the type of guanyl nucleotide occupying the nucleotide site on the protein. The affinity of this site for guanyl nucleotides is to a certain extent under hormonal control. Given a Ka of the site for guanyl nucleotides of 3 x 10^7 M in the presence of hormon

Beta-Oxidation of Odd and Even Carbon Fatty Acids

1 BCH-100 Fall 2007 BLH #13 (4 points) Question #1: (0.8 point) How many acetyl CoA's will come from a 12 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #2: (0.8 point) How many acetyl CoA's will come from a 13 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #3: (0.8 point) How many p

Fumarase Deficiency

Fumarase deficiency is an extremely rare disease in humans. People with this disease can survive although they are significantly affected. a) Given the importance of fumarase to the citric acid cycle how is it possible for people with fumarase deficiency to produce adequate ATP for survival? b) what would be the overall

Equilibrium Concentration from Keq and Initial Concentration

Part 1: what is equilibrium concentrations and concentration ratios of glucose-6-phosphate/glucose-1-phosphate in the phosphoglucomutase reaction when the initial concentration of glucose-6-phosphate is (a) .01 M and (b) 10^-3 M. The Keq for the reaction below is 19. Glucose-1-phosphate ---> glucose-6-phosphate Part 2: wh

Free Energy of Fructose-6-Phosphate and Glucose-6-Phosphate

Fructose-1,6-diphosphate can be converted to glucose-1-phosphate by three successive reactions: Fructose 1,6 diphosphate + H20 ---> fructose-6-phosphate + Pi Keq= 607 Fructose-6-phosphate ---> glucose-6-phosphate G°= -410 cal/mol Glucose-6-phosphate --->glucose-1-phosphate G°= 1750 cal/mol As

Acid/Base

You want to make a 0.1M acetate buffer from 0.1M solutions of acetic acid and acetate. How many mL of acetic acid would you need per liter of solution (with the rest of the volume coming from the acetate solution) to generate a pH of 4.75? The pKa of acetic acid is 4.76

Pka

The pKA of histidine in a protein active site where it is next to an aspartate or glutamate would be____________ the pKa of free histidine solution higher, lower, no different Is it higher??

Hydrolysis of Phosphocreatine

For the hydrolysis of phosphocreatine: Phosphocreatine + H2O==creatine + Pi the standard state deltaG is -42.8 kJ/mol at 37 degrees celsius. If in a given cell the concentration of creatine is 1.7mM, phosphate is 5.8mM, and phosphocreatine is 6.4mM, what is the deltaG for hydrolysis of phosphocreatine in the cell? Nearest 0.

Delta G

The equilibrium constant for the denaturation of a protein is determined to be 0.13 at 59.8 degrees Celsius. What is the standard state of delta G for the reaction, in kJ/mol to 0.01kJ/mol.

Phosphate System and Physiological pH

The phosphate system serves to buffer the intracellular fluid of cells at physiological pH because pK2 (7.20) lies near the normal pH range of 6.9-7.4. If the total cellular concentration of phosphate is 19mM and the pH is 6.9, what is the concentration of (H2PO4-) in mM?? Is this correct??? H2PO4-(aq) H+(aq) + HPO42-(aq)

Free energy and its affects

What affect does the presence of metal ions like Mg++ in the cell have on free energy of hydrolysis of ATP?? raise, lower, or no affect why??

Glycolysis and Gluconeogenesis

The following questions refer to the mitochondrial enzyme MALATE DEHYDROGENASE. What is the chemical reaction, within the citric acid cycle, that is catalyzed by malate dehydrogenase, and which substrates, products, and/or cofactors are involved? The reaction from the previous question is classified as a dehydrogenatio

Reaction process

Lactate dehydrogenase catalyzes the reversible reaction: Pyruvate + NADH + H^(+) --> Lactate + NAD^(+) Given the following facts, (a) tell in which direction the reaction will tend to go if NAD^(+), NADH, pyruvate, and lactate were mixed, all at 1 M concentrations, in the presence of lactate dehydrogenase at pH 7; (b) calc

Lipid Structures Multiple Choice Questions

See attached file for full problem description. Which structures contain saturated fatty acids or saturated fatty acid derivatives? Select all answers that apply. a. A b. B c. C d. D e. E Which structures contain unsaturated fatty acids or unsaturated fatty acid derivatives? Select all an

Trisaccharide and Sugar Properties

Provide the complete & accurate systematic name for this molecule. Be sure to include the correct names, in order, of the sugar residues, the correct numeric linkage for the glycosidic bonds, and any necessary information about anomeric carbons. See attached file for trisaccharide molecular formula. The first monosacchari

Scissile Bond

If the following peptide was the substrate for Carboxypeptidase A. Which of the indicated red arrows correctly identifies the scissile bond? See attached file for full problem description.

Catalytic Mechanism of Carboxypeptidase A

1) Carboxypeptidase A, a zinc-containing enzyme, hydrolyzes the C- terminal peptide bonds of polypeptides and/or proteins. In the enzyme- substrate complex, the zinc ion is coordinated to three enzyme side chains, the carbonyl oxygen of the scissile peptide bond, and a water molecule. A plausible model for the enzyme's reaction

Beta Glucosidase Activity Assay

I have followed the procedure that you provided to determine the activity of the beta glucosidase, however what I am unsure of is how the rate is calculated? In order for me to establish the rate, I followed your instructions however incubated the enzyme/buffer and PNPG solution for different times, ranging from 0-16 minut

Making a dilution

Make the following dilution: a working concentration of 100 ml 15 U/uL from an enzyme stock of 756,431 U/uL. Will you use a serial dilution? Why or Why not?

Effect on PAGE gel

Describe the effects the following would have on the way you PAGE gel would run: a) Increasing the percent of acrylamide b) Increasing the pH of the Tris-glycine buffer to 9.5 c) Decreasing the voltage at which the gel was run d) Increasing the voltage at which the gel was run by a factor of 3 e) Doubling the sample load vo