Experiment on Enzyme inhibition
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An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments are in the table
Glycogen (mM)
0.5, 1.0, 1.5, 2.0, 2.5, 3.0
Product Formed Glycogen only (mM/min)
22.6, 33.8, 38.0, 41.2, 44.8, 47.4
Product Formed Glycogen and Inhibitor (mM/min)
15.8, 24.9, 29.7, 35.1, 40.2, 44.9
What form or inhibition is used? Determine Km and Vmax?
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An experiment on enzyme inhibition is examined.
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Glycogen (mM)
0.5, 1.0, 1.5, 2.0, 2.5, 3.0
Product Formed Glycogen only (mM/min)
22.6, 33.8, 38.0, 41.2, 44.8, 47.4
Product Formed Glycogen and Inhibitor (mM/min)
15.8, 24.9, 29.7, 35.1, 40.2, 44.9
What form or inhibition is used? Determine Km and Vmax?
[S] Vo (Without Inhibitor) Vo (Without Inhibitor)
0.5 22.6 15.8
1.0 33.8 24.9
1.5 38.0 29.7
2.0 41.2 35.1
2.5 44.8 40.2
3.0 47.4 44.9
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