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Experiment on Enzyme inhibition

An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments are in the table

Glycogen (mM)
0.5, 1.0, 1.5, 2.0, 2.5, 3.0

Product Formed Glycogen only (mM/min)
22.6, 33.8, 38.0, 41.2, 44.8, 47.4

Product Formed Glycogen and Inhibitor (mM/min)
15.8, 24.9, 29.7, 35.1, 40.2, 44.9

What form or inhibition is used? Determine Km and Vmax?

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Glycogen (mM)
0.5, 1.0, 1.5, 2.0, 2.5, 3.0

Product Formed Glycogen only (mM/min)
22.6, 33.8, 38.0, 41.2, 44.8, 47.4

Product Formed Glycogen and Inhibitor (mM/min)
15.8, 24.9, 29.7, 35.1, 40.2, 44.9

What form or inhibition is used? Determine Km and Vmax?

[S] Vo (Without Inhibitor) Vo (Without Inhibitor)
0.5 22.6 15.8
1.0 33.8 24.9
1.5 38.0 29.7
2.0 41.2 35.1
2.5 44.8 40.2
3.0 47.4 44.9

The problem can ...

Solution Summary

An experiment on enzyme inhibition is examined.

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