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Experiment on Enzyme inhibition

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An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments are in the table

Glycogen (mM)
0.5, 1.0, 1.5, 2.0, 2.5, 3.0

Product Formed Glycogen only (mM/min)
22.6, 33.8, 38.0, 41.2, 44.8, 47.4

Product Formed Glycogen and Inhibitor (mM/min)
15.8, 24.9, 29.7, 35.1, 40.2, 44.9

What form or inhibition is used? Determine Km and Vmax?

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Glycogen (mM)
0.5, 1.0, 1.5, 2.0, 2.5, 3.0

Product Formed Glycogen only (mM/min)
22.6, 33.8, 38.0, 41.2, 44.8, 47.4

Product Formed Glycogen and Inhibitor (mM/min)
15.8, 24.9, 29.7, 35.1, 40.2, 44.9

What form or inhibition is used? Determine Km and Vmax?

[S] Vo (Without Inhibitor) Vo (Without Inhibitor)
0.5 22.6 15.8
1.0 33.8 24.9
1.5 38.0 29.7
2.0 41.2 35.1
2.5 44.8 40.2
3.0 47.4 44.9

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An experiment on enzyme inhibition is examined.

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Hypothetical experimental scenario or data

For this assignment, use your understanding of enzymes along with your scientific reasoning skills. This assignment provides you the opportunity to demonstrate your grasp of this module's concepts along with your capacity to interpret scientific data and draw conclusions from it.

A biologist is studying the behavior of a newly discovered enzyme. The optimum physical conditions for the enzyme's function are unknown. Likewise, nothing is known about the enzyme's potential substrates or inhibitors. Several experiments have been performed so far. Examine the graphs and present your conclusions about the behavior of this enzyme. Speculate on optimum conditions, substrates, coenzymes, inhibitors, etc. Use these data and your knowledge of enzymes to be as thorough as possible, but do not draw any conclusions that cannot be supported by the data.
(See attached file for data)

Write a short paper approximately 1-2 pages in length.

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