Absorbance and Concentration of Solution Before/After Dilution
Not what you're looking for?
A 5.0 mL aliquot of a stock tryptophan solution was diluted by adding 95 mL of water. The absorbance of that diluted solution was measured at 280 nm as 0.055 absorbance units. What is the concentration of the stock (original) solution? Hint: Make sure to calculate the dilution factor. (Molar extinction coefficient = 5579 cm-1 M-1.
Purchase this Solution
Solution Summary
This solution shows step-by-step calculations to determine the concentration of the stock solution using the dilution factor and molar extinction coefficient.
Solution Preview
A = ecl
0.055 = (5579 cm-1 M-1)(c)(1 cm)
c = 9.8 uM
Therefore, the diluted solution's ...
Purchase this Solution
Free BrainMass Quizzes
Hemophilia: Fact or Fiction
Do you know the truth about hemophilia? Test your knowledge here.
How Much Do You Know About Genetic Inheritance?
Most of us studied the basics of dominant and recessive genes at some point. However, genetics are much more complicated than this. How far beyond the basics does your knowledge go?
The Plant Body
This quiz will test your knowledge of the anatomy of a common plant.
How Well Do You Know Your Body?
This quiz will assess the different systems of the human body. It will examine everything from the organs to the cellular processes that occur.
Light and Sight Vocabulary
This quiz introduces basic definitions of vocabulary related to light and how human eyes. This information is important for an understanding of sight.