# dilute the stock solution

Please help with the following problem.

A spectrophotometric assay was to be carried out on a compound ε = 5000. The stock solution of standard had been made with a concentration of 0.0203M. Given a 1.000cm path length and a desired absorbance around 0.4-0.5, by what factor should the stock solution be diluted? (SHOW ALL MATH)

B) Exactly how would you best perform this dilution in the lab?

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#### Solution Preview

A spectrophotometric assay was to be carried out on a compound ε = 5000. The stock solution of standard had been made with a concentration of 0.0203M. Given a 1.000cm path length and a desired absorbance around 0.4-0.5, by what factor should the stock solution be diluted? (SHOW ALL MATH)

If we tried to measure the absorbance of the stock solution, we would have an enormously high absorbance reading which would be well beyond the limits of linearity for Beer's Law.

Beer's Law states that for dilute ...

#### Solution Summary

This solution shows how to dilute the stock solution in spectrochemistry. Step by step calculations are given in the answer.