1. Glycolysis occurs in the ________, the Krebs cycle occurs in the ________, and the electron transport chain occurs in the ________ membrane. A) mitochondria; mitochondria; mitochondrial B) cytoplasm; mitochondria; mitochondrial C) mitochondria; cytoplasm; plasma D) cytoplasm; cytoplasm; plasma E) cytoplasm; mitochondria;
See the attached .doc Copied from the document. See attachment for more details A) What is the initial velocity v0 called when the substrate concentration [S] >>>KM ([S] )? Label on graph. B) Simplify the above MM equation using the assumption that [S] >>>KM. From this simplified equation, what is the rate const
Experimental Procedure Question: Isolating and culturing individual olfactory sensory neurons (OSN). A procedure first describes a preparation of the cortical astrocyte feeder layer. Then, the isolation of OSNs from olfactory neuroepithelia is described.
Original posting as formulated by the Student: I am writing a research proposal in which I am attempted to describe an experimental procedure to isolate olfactory sensory neurons (OSNs) and analyze/compare the amino acid sequences of olfactory receptors expressed by individual OSNs. To do this, I am proposing isolating indivi
1. If your goal is to burn fat, you are better off exercising at lower intensity for longer periods of time, as opposed to short burts of more intense exercise. Looking at Figure 9.27(see attachment) explain why this is true on a cellular level. For number 3 can you check my reasoning here.. 3. In step 6 of citric acid cyc
Contained is a description of the signaling cascade that results from EGF stimulation of the Ras-MAPK pathway in cells. A paragraph description is included that tells in relative detail the various steps, and then a brief numbered list of events is given for quick reference in order to improve understanding of this complicated
What types of exposure and response are associated with DDT in humans?
The four phases of the disposition of a toxic compound to assess its toxicity are absorption through membranes in a system, the distribution throughout the system, the excretion and elimination from the system and lastly, the metabolic fate of the chemical. Using these four phases assess the toxicity on DDT. I would also like to
I am conducting an activity assay to assess the Enzymatic Hydrolysis of 4-Nitrophenyl ?-D-Glucopyranoside by ?-D-Glucosidase. I am happy with the assay, however one of the components is bovine serum albumin ( 0.2% solution in phosphate buffer, to which my enzyme solution is added). I initially assumed that the BSA acted as so
I have to give a 30 minute speech in a couple of weeks about a specific protein. The protein needs to have a crystal structure and have some type of societal impact. I would like a protein that is involved in sports or bodybuilding because that is the subject outside of school that I am most interested in. My question is basical
I need a summary and an explanation for this article: Factor VIII-von Willebrand Factor Complex Inhibits Osteoclastogenesis and Control Cell Survival, Received for publication, June 5, 2009, and in revised form, August 18, 2009 published. Marc Baud'huin, Laurence Duplomb, Stephane Teletchea, Celine Charrier, Mike Maillasso
Based on the attached data collected from DNA isolation from two different bacterial cultures: 1. Complete the table by determining the percentage content of the corresponding complementary bases for each bacterial population. On what assumptions are your calculations made? 2. Describe the relationship between Tm and base
A) Define "transamination" and "oxidative deamination" b) What is the role of the urea cycle, and what are the effects on an individual of a deficiency in one of the required enzymes?
I recently completed a experiment on SDS-Page electrophoresis to identify the molecular weight of my unknown proteins (unknown a and unknown b). I recently received my photograph that I took and I am having a hard time reading it. First off I am having difficult interpreting my photo as I only know that the 5th line is the marke
1. The stimulus for secretion of neurotransmitters into the synaptic cleft is an increase in A) sodium levels. B) calcium levels. C) neurotransmitter levels. D) chloride levels. E) potassium levels. 2. Multiple sclerosis is a result of A) the absence of myelin on nerve cells. B) having excess acetylcholine receptors.
An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments are in the table Glycogen (mM) 0
1. A drug impairs oxyegen consumption on liver mitochondria at a buffer of 7.4pH, Mg, and adp. It inhibits O2 uptake if isocitrate, 2-ketoglutarate and malate were substrates, but it will NOT is succinate is a substrate. Where's the site of inhibiiton: ATP synthase, malate dehydrogenase, Complex I, II, or III 2. Wha
Data from enzyme kinetic studies of an enzyme at specific substrate concentration. Data below also include from studies with town different inhibitors [S] (mM) Vo (uM/sec) Vo (uM/sec) Vo (uM/sec) w/o inhibitor w/inhibit A w/inhibit B 1.3 2.5
Please help solve the following dilutions problems with step by step calculations. 1) Prepare 100 mL of 5mM ascorbic acid with 35mM ascorbic acid solution 2) Calculate the final concentration when the 5mM is diluted to 1:500 If you wanted to make 100 mL of a 5mM ascorbic acid solution and you have on hand a 35mM ascorbic
In the movie, Lost in Space, spider-like creatures attacked members of the Robinson family as they explored an abandoned spaceship. Maureen (the mom) scans the creatures and reports that the spiders are silicon-based life-forms. (1) If silicon-based life exists, would it be able to consume carbon-based life, digest it, and u
During an experiment utilizing potatoes and the enzyme catalase, the heated potated exhibited less gas production when exposed to hydrogen peroxide. Explain why this happened?
Please see the attached file to help me draw the structures of the peptides mentioned.
Please help me with two of these questions. 9. Glucose is experimentally synthesized with C-14 at carbon 1, and used as a substrate for aerobic metabolism. Where is the label at the end of glycolysis? Draw the glycolysis product with the labeled carbon circled or highlighted. Now take that glycolysis product a little farther.
Please explain: An enzyme catalyzes a reaction at 20μmol/min when the substrate [s] is 0.01M. The Km for this substrate is 1 x 10-5M Assuming michaelis-menten kinetics are followed, what would the reaction velocity be when the concentration [s] is (a) 1 x 10-5 M (b) 1 x 10-6 M? Am I looking for Vo or Vmax or both? Pleas
Dear OTA Please see the attached file. Thanks in advance,
Describe the enzymatic activities used by cells to protect themselves from oxidative damage.
Conduction of protons by the F0 unit of ATP synthase is blocked by modification of a single side chain by dicyclohexylcarboiimide (DCCD). What are the most likely targets of action of this reagent? How might you use site-directed mutagenesis to determine whether this residue is essential for proton conduction?
14CH3-COOH is fed to microorganisms during an experiment. Trace the 14C through the citric cycle. How many ATP molecules can be generated from 1 mol of this substance? (The conversion of acetate to acetyl-CoA required the consumption of 2 ATP.)
What is the yield of ATP when each of the following substrates is completely oxidized to CO2 by a mammalian cell homogenate? Assume that glycolysis, the citric cycle and oxidative phosphorylation are fully active. a) Pyruvate b) NADH c) Fructose 1,6-biphosphate d) Glucose e) Dihydroxyacetone phosphate (Plea
Ethanol is oxidized in the liver to form acetate, which is converted to acetyl-CoA. Determine how many molecules of ATP are produced from 1 mol of ethanol. Note that 2 mol of NADH are produced when ethanol is oxidized to form acetate.
Please help with the attached file.