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Biological Chemistry

Cell Biology basic questions

1. Glycolysis occurs in the ________, the Krebs cycle occurs in the ________, and the electron transport chain occurs in the ________ membrane. A) mitochondria; mitochondria; mitochondrial B) cytoplasm; mitochondria; mitochondrial C) mitochondria; cytoplasm; plasma D) cytoplasm; cytoplasm; plasma E) cytoplasm; mitochondria;

Michaelis-Menton Equation and finding Vmax and Km from a graph

See the attached .doc Copied from the document. See attachment for more details A) What is the initial velocity v0 called when the substrate concentration [S] >>>KM ([S] )? Label on graph. B) Simplify the above MM equation using the assumption that [S] >>>KM. From this simplified equation, what is the rate const

Experimental Procedure Question: Isolating and culturing individual olfactory sensory neurons (OSN). A procedure first describes a preparation of the cortical astrocyte feeder layer. Then, the isolation of OSNs from olfactory neuroepithelia is described.

Original posting as formulated by the Student: I am writing a research proposal in which I am attempted to describe an experimental procedure to isolate olfactory sensory neurons (OSNs) and analyze/compare the amino acid sequences of olfactory receptors expressed by individual OSNs. To do this, I am proposing isolating indivi

Using Figure, explain why this is true on a cellular level...

1. If your goal is to burn fat, you are better off exercising at lower intensity for longer periods of time, as opposed to short burts of more intense exercise. Looking at Figure 9.27(see attachment) explain why this is true on a cellular level. For number 3 can you check my reasoning here.. 3. In step 6 of citric acid cyc

hydroylsis activity assay

I am conducting an activity assay to assess the Enzymatic Hydrolysis of 4-Nitrophenyl ?-D-Glucopyranoside by ?-D-Glucosidase. I am happy with the assay, however one of the components is bovine serum albumin ( 0.2% solution in phosphate buffer, to which my enzyme solution is added). I initially assumed that the BSA acted as so

Help on a Protein

I have to give a 30 minute speech in a couple of weeks about a specific protein. The protein needs to have a crystal structure and have some type of societal impact. I would like a protein that is involved in sports or bodybuilding because that is the subject outside of school that I am most interested in. My question is basical

Blood Factor Article Summary

I need a summary and an explanation for this article: Factor VIII-von Willebrand Factor Complex Inhibits Osteoclastogenesis and Control Cell Survival, Received for publication, June 5, 2009, and in revised form, August 18, 2009 published. Marc Baud'huin, Laurence Duplomb, Stephane Teletchea, Celine Charrier, Mike Maillasso

Relationship Between Tm and Base Pair Content

Based on the attached data collected from DNA isolation from two different bacterial cultures: 1. Complete the table by determining the percentage content of the corresponding complementary bases for each bacterial population. On what assumptions are your calculations made? 2. Describe the relationship between Tm and base

Definitions and Urea Cycle

A) Define "transamination" and "oxidative deamination" b) What is the role of the urea cycle, and what are the effects on an individual of a deficiency in one of the required enzymes?

SDS-Page electrophoreis to identify the molecular weight

I recently completed a experiment on SDS-Page electrophoresis to identify the molecular weight of my unknown proteins (unknown a and unknown b). I recently received my photograph that I took and I am having a hard time reading it. First off I am having difficult interpreting my photo as I only know that the 5th line is the marke

Cell Biology/Biochemistry questions

1. The stimulus for secretion of neurotransmitters into the synaptic cleft is an increase in A) sodium levels. B) calcium levels. C) neurotransmitter levels. D) chloride levels. E) potassium levels. 2. Multiple sclerosis is a result of A) the absence of myelin on nerve cells. B) having excess acetylcholine receptors.

Experiment on Enzyme inhibition

An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments are in the table Glycogen (mM) 0

Biochemistry: Liver Mitochondria Oxygen Uptake Inhibition

1. A drug impairs oxyegen consumption on liver mitochondria at a buffer of 7.4pH, Mg, and adp. It inhibits O2 uptake if isocitrate, 2-ketoglutarate and malate were substrates, but it will NOT is succinate is a substrate. Where's the site of inhibiiton: ATP synthase, malate dehydrogenase, Complex I, II, or III 2. Wha

Prepare 100 mL of 5mM Ascorbic Acid with 35mM Solution

Please help solve the following dilutions problems with step by step calculations. 1) Prepare 100 mL of 5mM ascorbic acid with 35mM ascorbic acid solution 2) Calculate the final concentration when the 5mM is diluted to 1:500 If you wanted to make 100 mL of a 5mM ascorbic acid solution and you have on hand a 35mM ascorbic

Silicon vs. Carbon Life Forms

In the movie, Lost in Space, spider-like creatures attacked members of the Robinson family as they explored an abandoned spaceship. Maureen (the mom) scans the creatures and reports that the spiders are silicon-based life-forms. (1) If silicon-based life exists, would it be able to consume carbon-based life, digest it, and u

Potato and enzyme catalase

During an experiment utilizing potatoes and the enzyme catalase, the heated potated exhibited less gas production when exposed to hydrogen peroxide. Explain why this happened?

Metabolic Pathways

Please help me with two of these questions. 9. Glucose is experimentally synthesized with C-14 at carbon 1, and used as a substrate for aerobic metabolism. Where is the label at the end of glycolysis? Draw the glycolysis product with the labeled carbon circled or highlighted. Now take that glycolysis product a little farther.

Michaelis-Menten Reactions

Please explain: An enzyme catalyzes a reaction at 20μmol/min when the substrate [s] is 0.01M. The Km for this substrate is 1 x 10-5M Assuming michaelis-menten kinetics are followed, what would the reaction velocity be when the concentration [s] is (a) 1 x 10-5 M (b) 1 x 10-6 M? Am I looking for Vo or Vmax or both? Pleas

DCCD

Conduction of protons by the F0 unit of ATP synthase is blocked by modification of a single side chain by dicyclohexylcarboiimide (DCCD). What are the most likely targets of action of this reagent? How might you use site-directed mutagenesis to determine whether this residue is essential for proton conduction?

Net ATP Generation From 14CH3-COOH

14CH3-COOH is fed to microorganisms during an experiment. Trace the 14C through the citric cycle. How many ATP molecules can be generated from 1 mol of this substance? (The conversion of acetate to acetyl-CoA required the consumption of 2 ATP.)

What is the yield of ATP

What is the yield of ATP when each of the following substrates is completely oxidized to CO2 by a mammalian cell homogenate? Assume that glycolysis, the citric cycle and oxidative phosphorylation are fully active. a) Pyruvate b) NADH c) Fructose 1,6-biphosphate d) Glucose e) Dihydroxyacetone phosphate (Plea

ATP Production From Oxidization Of Ethanol

Ethanol is oxidized in the liver to form acetate, which is converted to acetyl-CoA. Determine how many molecules of ATP are produced from 1 mol of ethanol. Note that 2 mol of NADH are produced when ethanol is oxidized to form acetate.