hydroylsis activity assay
Not what you're looking for?
I am conducting an activity assay to assess the Enzymatic Hydrolysis of 4-Nitrophenyl ?-D-Glucopyranoside by ?-D-Glucosidase. I am happy with the assay, however one of the components is bovine serum albumin ( 0.2% solution in phosphate buffer, to which my enzyme solution is added).
I initially assumed that the BSA acted as somesort of stabiliser to my enzyme, however I am unsure and would like this verified or corrected, and potentially explained how/why is plays this role and why it does not interfere (i hope!) with my enzyme and role!
Thanks
PS - I am not using the BSA as a control
Purchase this Solution
Solution Summary
A full solution is provided. The expert examines hydrolysis activity assay.
Solution Preview
Hi there,
first of all: your assumption is correct! Bovine Serum Albumin (BSA) is a protein stabilization reagent that is commonly employed in 0.1 to 1.0 % w/v solution in many biochemical assays.
Why is that? Well, I have not been able to find 'hard' scientific evidence that has examined the role of BSA in these experiments, however, one can deduce its role ...
Purchase this Solution
Free BrainMass Quizzes
How Much Do You Know About Genetic Inheritance?
Most of us studied the basics of dominant and recessive genes at some point. However, genetics are much more complicated than this. How far beyond the basics does your knowledge go?
The Plant Body
This quiz will test your knowledge of the anatomy of a common plant.
Birth 101
Do you know about childbirth? Find out with this quiz.
Infant Development: Sleep
How much do you know about infant sleep? Test your knowledge with this quiz.
Labor Comfort Measures
Do you know how to comfort someone in labor?