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    Bradford Assay Errors

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    What are the possible errors when the Bradford assay is used for the protein determination?

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    The Bradford protein assay is a colourimetric assay based upon a shift in the optimum absorbance of equilibrium forms of Coomassie Brilliant Blue G-250 dye (http://www.science.smith.edu/departments/Biochem/Biochem_353/Bradford.html). This dye binds to arginine and hydrophobic amino acid residues in proteins.

    In strong acid, the dye is doubly protonated and coloured red, but when bound to protein it is unprotonated and turns blue, which shifts the absorance maximum from 465 nm to 595 nm.. This colour change can be exploited using ...

    Solution Summary

    The solution discusses potential errors in the Bradford assay.