Purchase Solution

Bradford Assay Errors

Not what you're looking for?

Ask Custom Question

What are the possible errors when the Bradford assay is used for the protein determination?

Purchase this Solution

Solution Summary

The solution discusses potential errors in the Bradford assay.

Solution Preview

Please see the attached file.

Hi

The Bradford protein assay is a colourimetric assay based upon a shift in the optimum absorbance of equilibrium forms of Coomassie Brilliant Blue G-250 dye (http://www.science.smith.edu/departments/Biochem/Biochem_353/Bradford.html). This dye binds to arginine and hydrophobic amino acid residues in proteins.

In strong acid, the dye is doubly protonated and coloured red, but when bound to protein it is unprotonated and turns blue, which shifts the absorance maximum from 465 nm to 595 nm.. This colour change can be exploited using ...

Purchase this Solution


Free BrainMass Quizzes
Do You Know Your Macromolecules?

This quiz will assess your knowledge of the macromolecules that are important to living things.

Creating a Birth Plan

Preparing for a birth and want to make sure that you're including all the right information? Use this quiz to get on the right track and check your birth plan knowledge!

Biochemistry Basics

A refresher quiz to test your knowledge of basics concepts of biochemistry.

Identifying Variables in Science Experiments, Part 2

Using sample experiments, test yourself to see if you can identify independent, dependent, and controlled variables. Identifying variables is key in understanding and developing experiments. The questions are biology related, but this can be applied to any area of science.

BIOLOGY

Basics in biology