Can you help me with the following problem? You and your lab partner are responsible for making the buffer 1 M Tris-HCl pH 7.4. After much time, the closest you can get to pH 7.4 is 6.4. Your partner is trying to convince you that 1 pH unit is close enough. What is (or should be) your response?© BrainMass Inc. brainmass.com March 4, 2021, 10:22 pm ad1c9bdddf
to answer your question:
My response would be to try harder and work smarter. Instead you could use Tris base and not Tris-HCl and you would start off with a significantly more basic solution to pH. If this is not an option, you should continue to add base to get ...
This solution is comprised of an explanation of preparing buffers within the context of chemistry.