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Buffer Preparation - pH

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Can you help me with the following problem? You and your lab partner are responsible for making the buffer 1 M Tris-HCl pH 7.4. After much time, the closest you can get to pH 7.4 is 6.4. Your partner is trying to convince you that 1 pH unit is close enough. What is (or should be) your response?

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This solution is comprised of an explanation of preparing buffers within the context of chemistry.

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Hi there,

to answer your question:

My response would be to try harder and work smarter. Instead you could use Tris base and not Tris-HCl and you would start off with a significantly more basic solution to pH. If this is not an option, you should continue to add base to get ...

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