A step by step method to prepare a buffer with a specific pH and concentration is given. This includes guidelines to find the conjugate acid/base pair, calculation of the buffer ratio using the Henderson-Hasselbalch equation, and the determination of mass of chemicals reagents required to prepare the buffer.© BrainMass Inc. brainmass.com December 24, 2021, 7:53 pm ad1c9bdddf
See attached file for a specific example showing detailed calculations for preparing 1.50L of a 0.120 M phosphate buffer at pH=7.54.
Step 1: Look up table of Ka values for weak acids (available in the appendix of any general chemistry textbook). ...
The solution contains step-by-step instructions to prepare a buffer with a specific pH and concentration. The attached world file contains detailed calculation working through the preparation of a phosphate as a specific example.