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    Effect of pH on Mitochondria

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    If you isolate mitochondria and place them in buffer with a low pH, they begin to manufacture ATP. Why?

    © BrainMass Inc. brainmass.com December 24, 2021, 4:52 pm ad1c9bdddf
    https://brainmass.com/biology/cell-biochem/effect--ph-mitochondria-12604

    SOLUTION This solution is FREE courtesy of BrainMass!

    1. If you isolate mitochondria and place them in buffer with a low pH, they begin to manufacture ATP. Why?

    Permeability of outer membrane - Mitochondrial production of ATP requires a concentration gradient of H+, with a high concentration at the inter membrane space and a low concentration in the matrix. The inner membrane is impermeable to H+, but the outer membrane of the mitochondria will allow H+ to pass through.

    Thus, placing mitochondria in a low pH buffer produces a H+ gradient that can generate ATP through ATP synthetase.

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    © BrainMass Inc. brainmass.com December 24, 2021, 4:52 pm ad1c9bdddf>
    https://brainmass.com/biology/cell-biochem/effect--ph-mitochondria-12604

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