# Reaction rate and michaelis-menton kinetics

Not what you're looking for?

See the attached .doc

Copied from the document. See attachment for more details

A) What is the initial velocity v0 called when the substrate concentration [S] >>>KM ([S] )?

Label on graph.

B) Simplify the above MM equation using the assumption that [S] >>>KM. From this simplified equation, what is the rate constant that describes the MM kinetics in the region [S] >>>KM?

Indicate on graph.

1. Would this rate be useful in determining the catalytic efficiency of a biological enzyme under physiological conditions?

C) Simplify the above MM equation using assumption the [S] <<KM. From this simplified equation, what is the rate constant that describes the MM kinetics for [S] <<KM?

1. Would this rate be useful in determining the catalytic efficiency of a biological enzyme under physiological conditions?

D) Using the MM equation, what is true about the initial velocity v0 when KM = [S]?

Indicate on the graph.

This is the concentration at which one half of the enzyme's active sites are occupied by substrates.

##### Purchase this Solution

##### Solution Summary

In this solution a typical michaelis-menten question is posed with a graph, and the various aspects are to be labelled. Also, the equation is derived using different approximations (assuming substrate is much lower than Km, for example). Vmax and Km are identified on the graph, and several conceptual questions regarding enzyme kinetics are answered.

##### Solution Preview

A) This represents the Vmax, which would be the top dotted line on the graph

B) If [S] is much bigger than Km, we can essentially pretend Km is zero, so we can simplify

kcat*[E][S]/([S]+0)

=kcat*[E]

Again, this would put us in a concentration range that approaches the Vmax, since it is TOTALLY dependent on enzyme concentration!

This quantity would not be very ...

##### Purchase this Solution

##### Free BrainMass Quizzes

##### The Heart

This quiz test the understanding of the heart and some of its parts. It is important to understand how the heart functions and what makes it function.

##### Identifying Variables in Science Experiments, Part 2

Using sample experiments, test yourself to see if you can identify independent, dependent, and controlled variables. Identifying variables is key in understanding and developing experiments. The questions are biology related, but this can be applied to any area of science.

##### Cellular Respiration

This quiz is a review for cellular respiration.

##### Basic Immunology Quiz

Intro to immuno quiz. Covers the basics of immunology and recognition of foreign substances by the body.

##### The Transfer of Energy in an Ecosystem

This quiz will assess your knowledge of how energy is transferred in an ecosystem and the different levels of trophic organization.