Purchase Solution

Enzyme Kinetics

Not what you're looking for?

Ask Custom Question

The enzyme succinoxidase adds oxygen to succinate to give fumarate. The reaction follows Michaelis-Menton kinetics. The following rate data were determined for a solution in which the enzyme concentration is 10.0 uM:

[S](mM) ----------------- 0.33 10.00
vo (uM s-1) at 20 C ----- 0.50 1.17
vo (uM s-1) at 40 C ----- 2.41 5.65

(a) Determine Vmax and Km for the reaction at 20 C and 40 C.

For this question, I know that the Michaelis-Menton equation is:

vo = Vmax/1 + Km/[S]
- the equation then becomes:
vo = k2*[E]/ 1 + Km/[S]

We know what vo is for, [E],and [S], but k2 is not known. I'm not sure how to proceed with this question unless k2 is known.

(b) Compute the activation energy of this reaction (succinate + O2 --> fumarate).

For this question, would the equation K2/k1 = -Ea/R[1/T2 - 1/T1] be used. T2 and T1 is known, R is a constant (8.314) but how is k2 and k2 calculated. This part I do not understand.

(c) Will the activation energy be higher or lower in the absence of succinoxidase? Why?

Purchase this Solution

Solution Summary

This solution includes a rationale on how to answer all three parts of this question regarding enzyme kinetics. Based on what is known for parts a-c, guidance is given in terms of what equations to utilize to calculate certain values and how to interpret the question. All of this is done in about 250 words.

Solution Preview

Another form of the Michaelis-Menten equation is: vo/Vmax = [S]/(Km + [S])

In the (a) part, since you know vo at two different substrate concentrations, you can substitute these into the MM equation. Then you ...

Purchase this Solution

Free BrainMass Quizzes
General Chemistry - Classification of Matter

This test will assess your knowledge on the classification of matter which includes elements, compounds and mixtures.

Organic Chemistry Naming: Alkanes

This is a quiz which is designed to assist students with learning the nomenclature used to identify organic compounds. This quiz focuses on the organic compounds called Alkanes.


The quiz helps in revising basic concepts about thermochemistry.

Match Elements with their Symbols

Elements are provided: choose the matching one- or two-letter symbol for each element.

Functional groups in Organic Chemistry

You will be tested on the names of functional groups in Organic Chemistry. It is very important to know the functional groups to understand Organic reactions.