- Biological Chemistry
- Cell BioChem
- Enzyme, Catalysts and Reactions
I need help on how to approach this problem:
What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?
What is the ratio of substrate concentrations of a reaction proceeding at 90% and 10% of Vmax, respectively?
I know the basis of enzyme kinetics. I just don't know how to approach it. Do I use the Michaelis-Menten equation? and if so, how do I use it?
This Solution contains calculations to aid you in understanding the Solution to these questions.