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# Enzymes and Kinetics

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I need help on how to approach this problem:

What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?

What is the ratio of substrate concentrations of a reaction proceeding at 90% and 10% of Vmax, respectively?

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I know the basis of enzyme kinetics. I just don't know how to approach it. Do I use the Michaelis-Menten equation? and if so, how do I use it?

https://brainmass.com/biology/enzyme-catalysts-and-reactions/enzymes-kinetics-67005

#### Solution Preview

Cheers!

What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?

[S] = 10-3 M,

V0/Vmax = 25/100

As according to MM equation,

Vmax [S]
V0 ...

#### Solution Summary

This Solution contains calculations to aid you in understanding the Solution to these questions.

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## Understanding the Michaelis-Menten Equation, Enzyme Kinetics

For an enzyme which obeys Henri-Michaelis-Menten kinetics,
(1) At what substrate concentration will an enzyme characterized by a kcat of 30 s-1 and Km of 0.005 M show one quarter of its maximal rate?

(2) Calculate the fraction of Vmax that would be found when [S]=0.5 Km, [S]=3Km, and [S]=10Km

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