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    Enzymes and Kinetics

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    I need help on how to approach this problem:

    What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?

    What is the ratio of substrate concentrations of a reaction proceeding at 90% and 10% of Vmax, respectively?

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    I know the basis of enzyme kinetics. I just don't know how to approach it. Do I use the Michaelis-Menten equation? and if so, how do I use it?

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    Please see the attached file.
    Cheers!

    What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?

    [S] = 10-3 M,

    V0/Vmax = 25/100

    As according to MM equation,

    Vmax [S]
    V0 ...

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