# Enzymes and Kinetics

I need help on how to approach this problem:

What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?

What is the ratio of substrate concentrations of a reaction proceeding at 90% and 10% of Vmax, respectively?

-------

I know the basis of enzyme kinetics. I just don't know how to approach it. Do I use the Michaelis-Menten equation? and if so, how do I use it?

© BrainMass Inc. brainmass.com October 1, 2020, 6:24 pm ad1c9bdddfhttps://brainmass.com/biology/enzyme-catalysts-and-reactions/enzymes-kinetics-67005

#### Solution Preview

Please see the attached file.

Cheers!

What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?

[S] = 10-3 M,

V0/Vmax = 25/100

As according to MM equation,

Vmax [S]

V0 ...

#### Solution Summary

This Solution contains calculations to aid you in understanding the Solution to these questions.

$2.19