I need help on how to approach this problem:
I know the basis of enzyme kinetics. I just don't know how to approach it. Do I use the Michaelis-Menten equation? and if so, how do I use it?© BrainMass Inc. brainmass.com October 24, 2018, 7:25 pm ad1c9bdddf
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What is the Km of an enzyme if, at [S] = 10^-3 M, 25% of the maximum velocity of the reaction is obtained?
[S] = 10-3 M,
V0/Vmax = 25/100
As according to MM equation,
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Understanding the Michaelis-Menten Equation, Enzyme Kinetics
For an enzyme which obeys Henri-Michaelis-Menten kinetics,
(1) At what substrate concentration will an enzyme characterized by a kcat of 30 s-1 and Km of 0.005 M show one quarter of its maximal rate?
(2) Calculate the fraction of Vmax that would be found when [S]=0.5 Km, [S]=3Km, and [S]=10KmView Full Posting Details