You characterize a new enzyme's kinetics. Using a saturating (very high so all E is ES) substrate concentration, you measure the initial rate of the reaction at various enzyme concentrations. Does this data fit the Michaelis Menton model/equation? If not, what may be happening?
E (nM), rate (mM,s)
A. NO, the rate should only vary with substrate concentration, not enzyme concentration. The person collecting the data doesn't have a clue.
B. No, the activity does not change with increasing enzyme concentration correctly. The enzyme may be only active in a dimer that forms a high concentration.
C. Yes, it obeys the MM equation well.
D. No, the activity does not change with increasing enzyme concentration correctly. The enzyme may be inactive in a dimer that forms at high concentration
E. No, the rate should decrease as you increase the enzyme concentration. This reaction is not enzyme catalyzed.© BrainMass Inc. brainmass.com August 17, 2018, 1:10 pm ad1c9bdddf
In regards to enzyme concentration, as the enzyme concentration increases the rate of the reaction increases linearly, because there are ...
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