You characterize a new enzyme's kinetics. Using a saturating (very high so all E is ES) substrate concentration, you measure the initial rate of the reaction at various enzyme concentrations. Does this data fit the Michaelis Menton model/equation? If not, what may be happening?
E (nM), rate (mM,s)
A. NO, the rate should only vary with substrate concentration, not enzyme concentration. The person collecting the data doesn't have a clue.
B. No, the activity does not change with increasing enzyme concentration correctly. The enzyme may be only active in a dimer that forms a high concentration.
C. Yes, it obeys the MM equation well.
D. No, the activity does not change with increasing enzyme concentration correctly. The enzyme may be inactive in a dimer that forms at high concentration
E. No, the rate should decrease as you increase the enzyme concentration. This reaction is not enzyme catalyzed.© BrainMass Inc. brainmass.com December 24, 2021, 6:56 pm ad1c9bdddf
SOLUTION This solution is FREE courtesy of BrainMass!
In regards to enzyme concentration, as the enzyme concentration increases the rate of the reaction increases linearly, because there are more enzyme molecules available to catalyse the reaction. At very high enzyme concentration the substrate concentration may become rate-limiting, so the rate stops increasing. Normally enzymes are present in cells in rather low concentrations.
In this case we do not see that the rate at high enzyme concentration levels out. So the answer is D.