For an enzyme which obeys Henri-Michaelis-Menten kinetics,
(1) At what substrate concentration will an enzyme characterized by a kcat of 30 s-1 and Km of 0.005 M show one quarter of its maximal rate?
(2) Calculate the fraction of Vmax that would be found when [S]=0.5 Km, [S]=3Km, and [S]=10Km© BrainMass Inc. brainmass.com December 24, 2021, 4:41 pm ad1c9bdddf
Please refer to the attached file for a copy of this solution with equations, etc., formatted in Microsoft Word.
To solve this problem, you must first know the Henri-Michaelis-Menten equation.
where v=initial rate, Vmax=maximal velocity, [S]=concentration of substrate, and Km=Michaelis constant=concentration of substrate when ...
This solution shows an example of how to calculate common enzyme kinetic parameters (kcat, Km, Vmax, etc.), using the Henri-Michaelis-Menten equation. A step by step solution is provided.