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    Understanding the Michaelis-Menten Equation, Enzyme Kinetics

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    For an enzyme which obeys Henri-Michaelis-Menten kinetics,
    (1) At what substrate concentration will an enzyme characterized by a kcat of 30 s-1 and Km of 0.005 M show one quarter of its maximal rate?

    (2) Calculate the fraction of Vmax that would be found when [S]=0.5 Km, [S]=3Km, and [S]=10Km

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    Solution Preview

    Please refer to the attached file for a copy of this solution with equations, etc., formatted in Microsoft Word.

    To solve this problem, you must first know the Henri-Michaelis-Menten equation.

    where v=initial rate, Vmax=maximal velocity, [S]=concentration of substrate, and Km=Michaelis constant=concentration of substrate when ...

    Solution Summary

    This solution shows an example of how to calculate common enzyme kinetic parameters (kcat, Km, Vmax, etc.), using the Henri-Michaelis-Menten equation. A step by step solution is provided.