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    Finding Vmax and Km from [S] and Vobs Data

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    With the following info for the activity of an enzyme in the absence and presence of compound A, what is the: Km of the enzyme, the Vmax of the enzyme, and the action of compound A ( an inhibitor, stimulator, modulator, etc) and please describe why.
    S (mM) Vobs control Vobs with cmpd A
    0.1 0.68 0.91
    0.2 1.25 1.67
    0.5 2.50 3.33
    1 3.75 5.00
    2 5.00 6.67
    5 6.25 8.33
    10 6.82 9.09
    20 7.14 9.52

    keywords: Michaelis, Menten, equation

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    https://brainmass.com/biology/biological-chemistry/finding-vmax-km-vobs-data-148578

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    With the following info for the activity of an enzyme in the absence and presence of compound A, what is the: Km of the enzyme, the Vmax of the enzyme, and the action of compound A ( an inhibitor, stimulator, modulator, etc) and please describe why.
    S (mM) Vobs; ...

    Solution Summary

    The appropiate graphs are drawn to find Vmax and Km from [S] and Vobs data using Michaelis Menten kinetics. The effect of an added compound is also discussed.

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