Finding Vmax and Km from [S] and Vobs Data
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With the following info for the activity of an enzyme in the absence and presence of compound A, what is the: Km of the enzyme, the Vmax of the enzyme, and the action of compound A ( an inhibitor, stimulator, modulator, etc) and please describe why.
S (mM) Vobs control Vobs with cmpd A
0.1 0.68 0.91
0.2 1.25 1.67
0.5 2.50 3.33
1 3.75 5.00
2 5.00 6.67
5 6.25 8.33
10 6.82 9.09
20 7.14 9.52
keywords: Michaelis, Menten, equation
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Solution Summary
The appropiate graphs are drawn to find Vmax and Km from [S] and Vobs data using Michaelis Menten kinetics. The effect of an added compound is also discussed.
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With the following info for the activity of an enzyme in the absence and presence of compound A, what is the: Km of the enzyme, the Vmax of the enzyme, and the action of compound A ( an inhibitor, stimulator, modulator, etc) and please describe why.
S (mM) Vobs; ...
Purchase this Solution
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