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Determining Km and Vmax

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I have made a lineweaver-burk graph with some data, but I don't know how to determine Vmax and Km from it? Would you please give me an example of how to do it. Please make a lineweaver-graph with some values and tell me how to determine Km and Vmax. I can figure out the slope from the graph, but cannot figure out Y and x in Y = mx + b. See my graph attached. Here are some random values: 1/Vi (y) 1/[S]i (x)
166.666 .179115
175.000 .537345
233.334 1.07469
315.789 2.68817
456.521 3.58230
611.111 5.37346

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First of all, the graph you set up was not a Lineweaver-Burke plot.
To do one, you need to set up your graph to make a "XY Scatter Plot." I'm not sure what you did to make your graph, but it's not a graph of the data.
You want the 1/V on the y axis and 1[S] on the x axis.
Look at the attached Excel file. You will see the plot set up correctly. Notice that the x-axis goes from 0 to 6 ...

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I have made a lineweaver-burk graph with some data, but I don't know how to determine Vmax and Km from it? Would you please give me an example of how to do it. Please make a lineweaver-graph with some values and tell me how to determine Km and Vmax. I can figure out the slope from the graph, but cannot figure out Y and x in Y = mx + b. See my graph attached. Here are some random values: 1/Vi (y) 1/[S]i (x)

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Determine Km and Vmax and determine what types of inhibition

Data from enzyme kinetic studies of an enzyme at specific substrate concentration. Data below also include from studies with town different inhibitors

[S] (mM) Vo (uM/sec) Vo (uM/sec) Vo (uM/sec)
w/o inhibitor w/inhibit A w/inhibit B

1.3 2.5 1.17 0.62
2.6 4.00 2.10 1.42
6.5 6.3 4.0 2.65
13.0 7.6 5.70 3.12
26.0 9.0 7.20 3.58

Determine the Km and Vmax for the enzyme not inhibited. Plot all. What types of inhibition is displayed by inhibitor A and B? Would these inhibitors have an affinity for ES or E?

Everytime I plot this it is a curved line upside down. I do not know what I am doing wrong??? Please give me step by step. I am a very visual person.

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