Determining Km and Vmax
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I have made a lineweaver-burk graph with some data, but I don't know how to determine Vmax and Km from it? Would you please give me an example of how to do it. Please make a lineweaver-graph with some values and tell me how to determine Km and Vmax. I can figure out the slope from the graph, but cannot figure out Y and x in Y = mx + b. See my graph attached. Here are some random values: 1/Vi (y) 1/[S]i (x)
166.666 .179115
175.000 .537345
233.334 1.07469
315.789 2.68817
456.521 3.58230
611.111 5.37346
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Solution Summary
I have made a lineweaver-burk graph with some data, but I don't know how to determine Vmax and Km from it? Would you please give me an example of how to do it. Please make a lineweaver-graph with some values and tell me how to determine Km and Vmax. I can figure out the slope from the graph, but cannot figure out Y and x in Y = mx + b. See my graph attached. Here are some random values: 1/Vi (y) 1/[S]i (x)
Solution Preview
First of all, the graph you set up was not a Lineweaver-Burke plot.
To do one, you need to set up your graph to make a "XY Scatter Plot." I'm not sure what you did to make your graph, but it's not a graph of the data.
You want the 1/V on the y axis and 1[S] on the x axis.
Look at the attached Excel file. You will see the plot set up correctly. Notice that the x-axis goes from 0 to 6 ...
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