Explore BrainMass

# Km and Vmax: Derivation from 2 Substrates and 2 Vobs

This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

Can you help me derive the equations necessary to calculate Km and Vmax with a given observed velocity at two substrate concentrations. What is Vmax and Km if you know Substrate1 and Substrate2 and the matching Vobs1 and Vobs2?

Thank you for all your help.

https://brainmass.com/biology/biological-chemistry/km-vmax-derivation-substrates-vobs-148577

#### Solution Preview

Can you help me derive the equations necessary to calculate Km and Vmax with a given observed velocity at two substrate concentrations; aka, what is Vmax and Km if you know Substrate1 and Substrate2 and the matching Vobs1 and Vobs2?

This is essentially a two equation, two unknown type problem. Based on the Michealis-Menten equation...

Vobs1 = Vmax[s1] Eq 1
Km + [s1]

Vobs2 = Vmax[s2] Eq 2
Km + [s2]

Eq 1...isolate ...

#### Solution Summary

This solution outlines how to solve for Vmax an Km with variable substrate concentrations and Vobs.

\$2.19