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    Km and Vmax: Derivation from 2 Substrates and 2 Vobs

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    Can you help me derive the equations necessary to calculate Km and Vmax with a given observed velocity at two substrate concentrations. What is Vmax and Km if you know Substrate1 and Substrate2 and the matching Vobs1 and Vobs2?

    Thank you for all your help.

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    https://brainmass.com/biology/biological-chemistry/km-vmax-derivation-substrates-vobs-148577

    Solution Preview

    Can you help me derive the equations necessary to calculate Km and Vmax with a given observed velocity at two substrate concentrations; aka, what is Vmax and Km if you know Substrate1 and Substrate2 and the matching Vobs1 and Vobs2?

    This is essentially a two equation, two unknown type problem. Based on the Michealis-Menten equation...

    Vobs1 = Vmax[s1] Eq 1
    Km + [s1]

    Vobs2 = Vmax[s2] Eq 2
    Km + [s2]

    Eq 1...isolate ...

    Solution Summary

    This solution outlines how to solve for Vmax an Km with variable substrate concentrations and Vobs.

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