Km and Vmax: Derivation from 2 Substrates and 2 Vobs
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Can you help me derive the equations necessary to calculate Km and Vmax with a given observed velocity at two substrate concentrations. What is Vmax and Km if you know Substrate1 and Substrate2 and the matching Vobs1 and Vobs2?
Thank you for all your help.
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Solution Summary
This solution outlines how to solve for Vmax an Km with variable substrate concentrations and Vobs.
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Can you help me derive the equations necessary to calculate Km and Vmax with a given observed velocity at two substrate concentrations; aka, what is Vmax and Km if you know Substrate1 and Substrate2 and the matching Vobs1 and Vobs2?
This is essentially a two equation, two unknown type problem. Based on the Michealis-Menten equation...
Vobs1 = Vmax[s1] Eq 1
Km + [s1]
Vobs2 = Vmax[s2] Eq 2
Km + [s2]
Eq 1...isolate ...
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