Enzyme Kinetics: Hydrolysis Rate Data
An ATPase from yak saliva was isolated, and the following set of hydrolysis-rate data was obtained (see attachment). Find Km and Vmax.
© BrainMass Inc. brainmass.com October 24, 2018, 5:58 pm ad1c9bdddfhttps://brainmass.com/biology/cell-biochem/enzyme-kinetics-hydrolysis-rate-data-27306
Attachments
Solution Preview
There are two ways to do this, both relying on the equation:
1/v = (1/vmax) x (1 + Km/[S])
I've attached a spreadsheet with the two methods.
vmax is ...
Solution Summary
Solution can be found in two ways: plot v against [S] and fit a curve to it using Excel Solver or make the data fit a straight line.
$2.19
See Also This Related BrainMass Solution
ATP energy and calcium ion effect on muscles
Explain the relation between muscles, ATP energy, and the calcium ion.
I will be using for class, If there are any references please include them.
If there is any way one can provide specific examples as well related to the different relations I would really appreciate it.
Thank you
View Full Posting Details