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    Enzyme Kinetics: Hydrolysis Rate Data

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    An ATPase from yak saliva was isolated, and the following set of hydrolysis-rate data was obtained (see attachment). Find Km and Vmax.

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    Solution Preview

    There are two ways to do this, both relying on the equation:

    1/v = (1/vmax) x (1 + Km/[S])

    I've attached a spreadsheet with the two methods.

    vmax is ...

    Solution Summary

    Solution can be found in two ways: plot v against [S] and fit a curve to it using Excel Solver or make the data fit a straight line.