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Enzyme Kinetics: Hydrolysis Rate Data

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An ATPase from yak saliva was isolated, and the following set of hydrolysis-rate data was obtained (see attachment). Find Km and Vmax.

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Solution Preview

There are two ways to do this, both relying on the equation:

1/v = (1/vmax) x (1 + Km/[S])

I've attached a spreadsheet with the two methods.

vmax is ...

Solution Summary

Solution can be found in two ways: plot v against [S] and fit a curve to it using Excel Solver or make the data fit a straight line.

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