- Biological Chemistry
- Cell BioChem
Enzyme Kinetics: Hydrolysis Rate Data
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An ATPase from yak saliva was isolated, and the following set of hydrolysis-rate data was obtained (see attachment). Find Km and Vmax.
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There are two ways to do this, both relying on the equation:
1/v = (1/vmax) x (1 + Km/[S])
I've attached a spreadsheet with the two methods.
vmax is ...
Solution can be found in two ways: plot v against [S] and fit a curve to it using Excel Solver or make the data fit a straight line.