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Constructing kinetics plots for galactose uptake

I am uncertain how to approach this problem:

uptake of galactose into red blood cells was looked at: the data is as follows,

galactose concentration mmol/l ^-1 galactose uptake(j)/nmol mg ^-1 protein min

x absent x present

0.100 4.00 2.27
0.125 4.69 2.72
0.250 7.09 4.59
0.500 9.62 6.94
1.000 11.49 9.50

rate of inward uptake (j)
x is thought to be an inhibitor of transport systems.

- from this data i need to work out the appropiate values and construct Hofstee-Eadie plots for the uptake of galactose both with and without the presence of X and put both plots on the same axes.

-Then i need to work out the values of j^max and K^t for the uptake of galactose, with and without (X), place in a table and explain the values obtained.

- I need to write a word equation for when galactose is phosphorylated by an enzyme in a red blood cell. I think that galactose plus inorganic phosphate in the presence of galactokinase would yield galactose - 1- phosphate. Would this be acceptable as a word equation?

Solution Preview

Please see the attached file for the work done to achieve the values given (the red line is with the inhibitor. The ...

Solution Summary

In this solution we explore the development of a kinetics model from experimental data. Specifically, when we know the data for a metabolic process (in this case, galactose uptake), how do we construct an eadie-hofstee plot? Also the maximal rates and the other pertinent constants are derived from this information. An excellent primer on enzyme kinetics!