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    KM of a Michaelis-Menten enzyme

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    The KM of a Michaelis-Menten enzyme for a substrate is 1.0 x 10-4 M. At a substrate concentration of 0.2 M, Vo=43x10^-6 M for a certain enzyme concentration. However, with a substrate concentration of 0.02 M, Vo has the same value.
    (A) Using numerical calculations show that this observation is accurate.
    (B) What is the best range of [S] for measuring KM?

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    Solution Preview

    Please see the attachment.(A) Km = 1.0 x 10-4M
    [S] = 2 x 10-1M
    Vo = 43 x 10-6M
    Using Michaelis-Menten equation:
    Vo = Vmax[S]
    Km + [S]
    At [S] = 2 x 10-1M,
    43 x ...

    Solution Summary

    This solution involves step by step calculations for finding the KM for a Michaelis-Menten enzyme