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Understanding enzyme kinetics - Henri-Michaelis-Menten model

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An enzyme with a Km of 0.36 mM was assayed using substrate concentrations of 3 x 10E-7 M, 9.5 x 10E-5 M, 1.5 x 10E-4, 1.16x10E-3, 3 x 10E-3 M, 0.01 M, 0.08 M. At a substrate concentration of 0.08 M, the initial velocity was 192 nM/min. Calculate the initial velocity at each of the other substrate concentrations. Graph the calculated velocities using the Henri-Michaelis-Menten model.

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Solution Summary

The solution contains a complete description of how to approach this type of enzyme kinetics question. The Henri-Michaelis-Menten equation is explained, and each of the variables in the equation are defined. The solution also contains several important tips for avoiding common mistakes, including unit analysis.

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Problem:

An enzyme with a Km of 0.36 mM was assayed using substrate concentrations of 3 x 10E-7 M, 9.5 x 10E-5 M, 1.5 x 10E-4, 1.16x10E-3, 3 x 10E-3 M, 0.01 M, 0.08 M. At a substrate concentration of 0.08 M, the initial velocity was 192 nM/min. Calculate the initial velocity at each of the other substrate concentrations. Graph the calculated velocities using the Henri-Michaelis-Menten model.

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