1) Glycerol can enter the glycolysis pathway by being converted to what? 2) How many molecules of ATP are used up in the glycolysis pathway? 3) How many pyruvate molecules are obtained from one molecule of glucose in the glycolysis pathway? 4) How many molecules of ATP are produced in the glycolysis pathway? 5) H
If you add 1 drop of 5% bromine solution in hexane to 1mL each of 5% solution of lard, olive oil, and corn oil dissolved in hexane, what happens to the bromine that causes each mixture to turn colorless? If you continue adding drops of bromine to each, eventually the lipids change color. For our experiment it took 14 for lard
1) Since calcium and magnesium salts of fatty acids are insoluble in water, how would you expect this to affect the usefulness of soap in hard water? 2) If you were to make soap (using 4 grams of lard, 2 mL of 7.8 M sodium hydroxide & boiling it) and add it to the following and inverting, what would you expect to see as far
3. What are the assumptions of the Monod-Wyman-Changeux (WMC) model of allosteric transitions?
What is the evidence that fructose bisphosphate aldolase is a Class I Aldolase?
3 . Describe the role of each member of the "triad" in the mechanism of chymostrypsin. 4. Some enzymes show a bell shaped curve for enzyme activity versus pH. What conclusions can you reach about the nature and the function of groups in the enzyme? Name an enzyme which exhibits this characteristic. 7. In 1935, two famous
You are studying a biochemical pathway in the bread mold NEUROSPORA crassa that is involved in the production of the compound C. You know that compounds A,B,D,E,F are required in this pathway, but you do not know the order in which they are synthesized. In order to study this, you isolate five auxotrophic strains that cannot syn
I need some help with this challenging question: 13C-NMR spectroscopy was used to detect (13)C-label accumulation in cerebral metabolites following the intravenous infusion of [1,6-(13)C]-glucose (Glc). The (1)H- [(13)C]-NMR method yielded high-quality (1)H-[(13)C]-NMR spectra. As a result, the (13)C labeling of [4-(13)C]-glut
The relative metabolic activities in an organism of glycolysis + the citric acid cycle vs. the pentose phosphate pathway + gluconeogenesis can be measured by comparing rates of 13CO2 generation upon administration of glucose labeled with 13C at C1 with that of glucose labeled at C6. Explain why this is possible.
Two intracellular molecules X and Y are both normally synthesized at a constant rate of 1000 molecules per second per cell. Molecules X is broken down slowly: each molecule of X survives on average for 100 seconds, Molecule Y is broken down 10 times faster, each molecule of Y survices on average for 10 seconds. a. calcu
Ethanol in the body is oxidized to acetaldehyde by liver alcohol dehydrogenase (LADH). Other alcohols are also oxidized by LADH. For example, methanol, which is mildly intoxicating, is oxidized by LADH to the quite toxic product formaldehyde. The toxic effects of ingesting methanol (a component of many commercial solvents) can
(See attached file for diagrams) 1. For a Michaelis-Menten reaction, k1 = 5 x 107 M-1 s-1, k-1 = 2 x 104 s-1, and k2 = 4 x 102 s-1. Calculate Ks and Km for this reaction. Does substrate binding achieve equilibrium or the steady state? 2. At what substrate concentration will an enzyme having a kcat of 30 s-1 and a Km of 0
The activity of an enzyme was determined in the presence and absence of a suspected inhibitor. You can see the activity data in the attached Word document. Plot the data using the double reciprocal plot (Lineweaver-Burk) to determine whether the suspected inhibitor acts noncompetitively, competitively, or uncompetitively. Cal
The KM of a Michaelis-Menten enzyme for a substrate is 1.0 x 10-4 M. At a substrate concentration of 0.2 M, Vo=43x10^-6 M for a certain enzyme concentration. However, with a substrate concentration of 0.02 M, Vo has the same value. (A) Using numerical calculations show that this observation is accurate. (B) What is the best
Here is the problem: 1. Assume you have a solution containing 0.1 mol of Ala adjusted to pH = 0.5 with HCl. You begin adding 1.0 M NaOH. Plot (do not sketch) the resulting titration curve (pH vs. moles NaOH added) showing all inflection points. Show your calculations and be sure to state your assumptions. 2. Plot a simil
Can someone check my answers to the following, please make any corrections and explanations as needed.... N---Tyr---Lys---Thr---C pka 9.1 10.1 10.5 13 2.6 ph2 +1 0 +1 0 0 =+2 ph6 +1 0 +1 0 -1 =+1 ph13 0 -1 0 0 -1 =-2 N---Asp---Met---Ala---C
(See attached file for full problem description) --- 9. An organism of unknown origin produces a potent inhibitor of nerve conduction which you wish to sequence. Amino acid analysis shows the peptide's composition to be 5 Ala, 1 Lys, 1 Phe. Reaction of the intact peptide with phenylisothiocyanate (Edman's reagent) followed
(See attached file for full problem description) --- For problems 6-8, assume standard conditions of pH 7 and 25C, unless otherwise stated, and assume equilibrium constants in which any [H+] and [H2O] terms have been incorporated into the constant, i.e., you don't have to use [H+] in any of the calculations. 6. Th
MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) In absence of oxygen, a yeast cell undergoes fermentation and uses 100 molecules of glucose. How much net ATP will be generated? A) 100 B) 36 C) 400 D) 300 E) 200 2) All the following statements pertai
List three fates of pyruvic acid.
Glucose and sucrose easily produced alcohol fermentation in yeast. Why didn't galactose and lactose? This was the virtual experiment. pH was set at 7 temperature 20 degrees C 20 ml of glucose was added to 1ml yeast 20ml or sucrose was added to 1ml of yeast 20ml of galactose was added to 1 ml of yeast 20ml of lact
Why can cells function so efficiently under anaerobic conditions? I don't understand any of this. Please help
The E-amino group of lysine has a pka of 10.5: a) What fraction of these groups will be protonated in a dilute solution of lysine at pH 9.5? b) At pH 11.0?
I need some help answering this questsion: If I had 1L of a 1.0M solution of glycine at the isoelectric pH (6.02) and I added 0.3 mol of HCL, what would be the new pH? What would be the pH if I added 0.3 mol of NaOH instead of the acid?
I need some help with biochemical questions: 1. How do you calculate the pH of a solution of lysine if the side chain amino group is 3/4 dissociated? 2. What is the isoelectric point of His? 3. For L-Ile, describe with drawings why it is 2S,3S? 4. What are the 6 atoms that are coplanar due to the resticted rotation about
--Which non covalent interactions are most likely to be important in protein-protein interactions in aqueous environment? --What is the driving force for proteins that are embedded in lipid membranes?
Carbohydrate production is known as ___?
One tube with elodea produces gass bubbles. What gas is released while the plant is making food?
Give the name for the system that readily accepts electrons from the chlorophyll molecules.
The process in which plants make their food is ___?