Consider this peptide: Phe-Glu-Ser-Met What is the net charge of this peptide at ph=1 What is the net charge of this peptide at ph=7
Mycoplasmas are the smallest living cells. They are spherical and have a diameter of about 0.33 μm (radius of mycoplasmas = 0.165 μm)a. (a)Glucose is the major energy source of mycoplasma cells. Its concentration within such cells is 0.1 mM. Calculate the number of glucose molecules within a single cell. (b) The fi
Which of these apply to Enzymes A. The majority of chemical reactions in our bodies do not require enzymes. B. Enzymes are functional proteins C. Enzymes get used up as part of a reaction D. Cyto chrome P-450 is an example of an enzyme that helps to oxidize chemicals in the body. E. Biotransformation of toxins could not o
A friend insists that all mutagens are dangerous. How would you respond?
I carried out the practical shown in the attachment, and am struggling with the calculations, Can anyone please explain what I need to do? As there are different calculations for different bits I am submitting separate post for each bit. Can anyone please show me how to determine carbon dioxide produced? The model answers f
I carried out the practical shown in the attachment, and am struggling with the calculations, Can anyone please explain what I need to do? As there are different calculations for different bits I am submitting separate post for each bit. Is there anyone who can show my how to determine ethanol formed? The model answers from
I have made a lineweaver-burk graph with some data, but I don't know how to determine Vmax and Km from it? Would you please give me an example of how to do it. Please make a lineweaver-graph with some values and tell me how to determine Km and Vmax. I can figure out the slope from the graph, but cannot figure out Y and x in Y
Which fatty acid -palmitic, stearic, palmitoleic, or arachidonic, would have lowest m.p. and explain why. Which would be chiral? A)glyceryl tripalmitate,B) glyceryl 1-stearate, 2,3 dipalmitate,C) glyceryl 2-stearate, 1,3-dipalmitate. Explain why. A lipid is isolated and treated with boiling aqueous NaOH. The products ob
I have a series of protein free patient blood samples at time; 0, 30, 60, 90, 120, 180 mins. I also have a standard sample. I have measured the absorbance of these samples; 0.655, 0.717, 0.689, 0.430, 0.852, 0.477 respectively. Standard sample 0.760 The glucose concentration in the standard test tube is 0.278 mmole/L. Using Beer
Question #1: (1 point) In the urea cycle, the molecule that is synthesized in the cytosol and transported to the mitochondrial matrix for subsequent reactions is a.) citrulline b.) ornithine c.) argininosuccinate d.) aspartate e.) carbamoyl-phosphate Question #2: (1 point) Inosine 5'-phosphate is a precursor to which
The standard reduction potential (Eo') for the reduction of fumarate is +0.03V. The Eo' for the reduction of FAD in a given protein that accepts the electrons from succinate is -0.40V. Given the reaction: succinate + FAD==fumarate + FADH2 Caluculate Eo' for the reation
Guanyl nucleotide regulatory proteins (N) alter cell function based on the type of guanyl nucleotide occupying the nucleotide site on the protein. The affinity of this site for guanyl nucleotides is to a certain extent under hormonal control. Given a Ka of the site for guanyl nucleotides of 3 x 10^7 M in the presence of hormon
Problem: How much energy is needed for the Na/K pump to move 3 Na out and 2 K in when Nai (Na inside)= 9.2mM, Nao (outside)= 115, Ki (K inside)= 135mM and Ko (K outside)= 3mM? Include the effects of membrane potential; it will have to be calculated based on the given ion concentrations and PNa/Pk of 0.013.
Calculate the pH gradient across the inner mitochondrial membrane that is necessary to drive the ATPase reaction in the direction of ATP synthesis under steady state conditions at 25°C where ATP=10^-5 M ADP= 10^-3 M Pi=2 x 10^-2 M Also- do not ignore the effect of the membrane potential!
1 BCH-100 Fall 2007 BLH #13 (4 points) Question #1: (0.8 point) How many acetyl CoA's will come from a 12 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #2: (0.8 point) How many acetyl CoA's will come from a 13 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #3: (0.8 point) How many p
Lineweaver-Burk Plot from Absorbance Data (with and without inhibitor) and Michaelis-Menten Kinetics
I carried out a practical last week and need to write it up but canot remember how to do the calculations from the Michaelis-Menten graph plotted which I guess helps me to plot a Lineweaver-Burk. I have attached the practical requirements. I have to produce a table (attached) but cannot fill in certain columns until I can work
Although animals do not have glyoxylate cycle its is still possible to find radioactively labelled carbon atoms from triacylglycerols incorporated into glucose. What are the reasons for this to occur? And if we have to make a flow diagram to show this...how will the series of reaction take place.
Fumarase deficiency is an extremely rare disease in humans. People with this disease can survive although they are significantly affected. a) Given the importance of fumarase to the citric acid cycle how is it possible for people with fumarase deficiency to produce adequate ATP for survival? b) what would be the overall
The following kinetic data was obtained using a substrate and an inhibitor. Find Km and Vmax for the substrate in mM and mM/min, then determine the type of inhibition and determine the Ki or Ki's. [S] mM [I] mM Rate mM/min 10 0 600 20 0 667 30 0 692 40 0 706 50 0 714 60 0 720 10000 0 750 10 30 273 20 30 400 30 30 4
With the following info for the activity of an enzyme in the absence and presence of compound A, what is the: Km of the enzyme, the Vmax of the enzyme, and the action of compound A ( an inhibitor, stimulator, modulator, etc) and please describe why. S (mM) Vobs control Vobs with cmpd A 0.1 0.68 0.91 0.2 1.25 1.67 0.5
Can you help me derive the equations necessary to calculate Km and Vmax with a given observed velocity at two substrate concentrations. What is Vmax and Km if you know Substrate1 and Substrate2 and the matching Vobs1 and Vobs2? Thank you for all your help.
Part 1: what is equilibrium concentrations and concentration ratios of glucose-6-phosphate/glucose-1-phosphate in the phosphoglucomutase reaction when the initial concentration of glucose-6-phosphate is (a) .01 M and (b) 10^-3 M. The Keq for the reaction below is 19. Glucose-1-phosphate ---> glucose-6-phosphate Part 2: wh
Explain the four major macromolecules found in a living organism and how they are used by a living organism.
Why is the element carbon so important to biology and why does its chemistry allow for the complicated chemistry involved in living systems.
You want to make a 0.1M acetate buffer from 0.1M solutions of acetic acid and acetate. How many mL of acetic acid would you need per liter of solution (with the rest of the volume coming from the acetate solution) to generate a pH of 4.75? The pKa of acetic acid is 4.76
The pKA of histidine in a protein active site where it is next to an aspartate or glutamate would be____________ the pKa of free histidine solution higher, lower, no different Is it higher??
For the hydrolysis of phosphocreatine: Phosphocreatine + H2O==creatine + Pi the standard state deltaG is -42.8 kJ/mol at 37 degrees celsius. If in a given cell the concentration of creatine is 1.7mM, phosphate is 5.8mM, and phosphocreatine is 6.4mM, what is the deltaG for hydrolysis of phosphocreatine in the cell? Nearest 0.
The equilibrium constant for the denaturation of a protein is determined to be 0.13 at 59.8 degrees Celsius. What is the standard state of delta G for the reaction, in kJ/mol to 0.01kJ/mol.
The phosphate system serves to buffer the intracellular fluid of cells at physiological pH because pK2 (7.20) lies near the normal pH range of 6.9-7.4. If the total cellular concentration of phosphate is 19mM and the pH is 6.9, what is the concentration of (H2PO4-) in mM?? Is this correct??? H2PO4-(aq) H+(aq) + HPO42-(aq)
What affect does the presence of metal ions like Mg++ in the cell have on free energy of hydrolysis of ATP?? raise, lower, or no affect why??