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Agarose gel electrophoresis

When digested DNA is analysed using agarose gel electrophoresis the total size of fragments sometimes does not add up to the size of the original DNA molecule. How can this be explained and how can this be seen in ethidium bromide stained agarose gels?

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This can happen when digestion by restriction endonucleases does not carry on to completion. It that case some of the original sized DNA can still be seen in agarose gels. Another confusing ...

Solution Summary

When DNA is digested with restriction endonucleases and analysed using agarose gel electrophoresis - we expect the size of the digested fragments to add up the the size of the original DNA. However, sometimes they don't!

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