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    Enzyme//Inhibitor

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    The following kinetic data was obtained using a substrate and an inhibitor. Find Km and Vmax for the substrate in mM and mM/min, then determine the type of inhibition and determine the Ki or Ki's.

    [S] mM [I] mM Rate mM/min
    10 0 600
    20 0 667
    30 0 692
    40 0 706
    50 0 714
    60 0 720
    10000 0 750
    10 30 273
    20 30 400
    30 30 474
    40 30 522
    50 30 556
    60 30 581
    10000 30 749
    10 60 176
    20 60 286
    30 60 360
    40 60 414
    50 60 455
    60 60 486
    10000 60 748
    10 100 120
    20 100 207
    30 100 273
    40 100 324
    50 100 366
    60 100 400
    10000 100 746

    © BrainMass Inc. brainmass.com April 3, 2020, 5:11 pm ad1c9bdddf
    https://brainmass.com/biology/biological-chemistry/enzyme-inhibitor-149186

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    Solution Preview

    Please open up the Excel file that I attached.

    The first thing I did was to transfer the data from the Word document into Excel. No problem there.
    Then, I reformatted the data so that it looked uncluttered. Essentially, remove all formatting for the cells.

    Next, we want to obtain double-reciprocal data so that we can plot and analyze the data easily. This is important so that we can create a Lineweaver-Burke plot. Therefore, you will see that I created two new columns, 1/[S] and 1/rate.

    Then we create a plot to visualize the data.

    To do this, highlight everything from D2 to E8. Make sure you understand this. Start your highlighting in D2 and go to E8. You should have a block of 14 cells highlighted.

    Once you've done that, go to Chart Wizard and select XY (Scatter) and select the subtype with no connecting line. All you want is the data points. Click "Finish."

    You will see a preliminary graph for the data set with no inhibitor present.

    Right click on ...

    Solution Summary

    The expert examines enzyme and inhibitors. The type of inhibition and Ki and Ki's are determined.

    $2.19

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