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    Enzyme inhibitor in a reaction

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    Five reaction mixtures containing equal concentrations of an enzyme are prepared to the substrate concentrations indicated in the table below, and the initial rates are measured. The experiment is then repeated with an enzyme inhibitor present at a concentration of 2.2 x 10-4 M in each reaction mixture. By use of Lineweaver-Burke plots (or other plots) of these data, graphically determine KM for the substrate, KI for the inhibitor, and Vmax in the presence and absence of inhibitor. Is this a competitive or noncompetitive inhibitor?

    *Note: I have included a small table with this problem as an attachment. It should be straight forward; please let me know if you have any problem opening it.

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    Solution Summary

    This post includes a solution to an enzyme inhibitor problem which includes a quick plotting of a curve.