Explore BrainMass
Share

Explore BrainMass

    Treatment with carboxypeptidase of a peptide sequence

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    I have a peptide sequence with no prolines, 2 positively charged residues at the c-terminal ends of 2 fragments created by trypsin cleavage, but shows no fragments from carboxypeptidases A or B. I thought if a positively charged residue was the c-terminal end of the primary peptide, then I should have seen fragments from carboxypeptidase B, since there is no blocking proline by either the Arg or Lys. I am stumped--what am I missing?

    © BrainMass Inc. brainmass.com October 10, 2019, 2:18 am ad1c9bdddf
    https://brainmass.com/biology/biological-chemistry/treatment-carboxypeptidase-peptide-sequence-371181

    Solution Preview

    Carboxypeptidase B cleaves away the last amino acid of your sequence if it is basic. Since you saw no fragments ...

    Solution Summary

    In this solution a proteolysis scheme using carboxypeptidases A and B is used to determine properties of a peptide. This is in an effort to help determine the protein sequence

    $2.19