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Peptide Sequencing

Deducing a peptide sequence from enzymatic and chemical cleavage.

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GRE subject Question
You have isolated and purified a a small octapeptide protein and want to determine its primary structure. Deduce the sequence from the following experimental results.

A) Complete amino acid analysis, corrected for amino acid hydrolysis loss yielded the following composition (Arg, Glu, Lys, Met, Ser, Thr, Trp, Tyr)

B) Reduction with Dansyl Chloride followed by acid hyrolysis and separation yielded the following dansylated compound.: ...

Solution Summary

The solution determines the peptide sequencing from the enzymatic and chemical cleavage.