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DCCD and the F0 unit of ATP synthase

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Conduction of protons by the F0 unit of ATP synthase is blocked by modification of a single side chain by dicyclohexylcarboiimide (DCCD). What are the most likely targets of action of this reagent? How might you use site-directed mutagenesis to determine whether this residue is essential for proton conduction?

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Solution Summary

site-directed mutagenesis could be used to study which residue is essential for proton conduction by the F0 unit of ATP synthase.

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DCCD is dicyclohexylcarbodiimide, a chemical reagent which reacts with acidic amino acid residues. This means that the most likely targets for this reagent are aspartic acid and glutamic acid residues, the two acidic amino acids that are part of proteins.

Using site directed mutagenesis means making mutations of the ATP synthase gene and changing ...

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