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Oxidative Phosphorylation and Uncoupling

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1. If succinate is used as the electron donor in oxidative phosphorylation, how many ATPs should be generated for each pair of electrons donated by succinate?

2. Respiration is coupled to ATP synthesis. What does UNCOUPLING mean?

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The solution discusses a response to a problem regarding oxidative phosphorylation and uncoupling.

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1. If succinate is used as the electron donor in oxidative phosphorylation, how many ATPs should be generated for each pair of electrons donated by succinate?

Answer:

Introduction to Oxidative phosphorylation:
The free energy available as a consequence of transferring 2 electrons from NADH. Oxidative phosphorylation traps this energy as the high-energy phosphate of ATP. In order for oxidative phosphorylation to proceed, two principal conditions must be met. First, the inner mitochondrial membrane must be physically intact so that protons can only re-enter the mitochondrion by a process coupled to ATP synthesis. Second, a high concentration of protons must be developed on the outside of the inner membrane.
A. During oxidative phosphorylation the proton gradient generated by the electron transport chain drives the formation of ATP from ADP
B. Due to the high concentration of protons on one side of the membrane the protons are ...

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