Explore BrainMass
Share

Explore BrainMass

    Net ATP Generation From 14CH3-COOH

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    14CH3-COOH is fed to microorganisms during an experiment. Trace the 14C through the citric cycle. How many ATP molecules can be generated from 1 mol of this substance? (The conversion of acetate to acetyl-CoA required the consumption of 2 ATP.)

    © BrainMass Inc. brainmass.com October 9, 2019, 9:01 pm ad1c9bdddf
    https://brainmass.com/biology/biological-chemistry/net-atp-generation-170137

    Solution Preview

    We have to trace the label of 14CH3COOH through the Krebs cycle.
    Here's how:

    14CH3COOH -----> 14CH3CO-CoA

    14CH3CO-CoA + OAA ----> citrate

    Where is the label in citrate? Here is its structure:

    14CH2-COOH
    |
    HO-C-COOH
    |
    CH2COOH

    Then citrate gets dehydrated by aconitase to form cis-aconitate:

    14CH2-COOH
    |
    C-COOH
    ||
    CHCOOH

    Then it gets hydrated to form isocitrate:

    14CH2-COOH
    ...

    Solution Summary

    The expert calculates the ATP generated from an organism metabolizing 14CH3-COOH. The conversion of acetate to acetyl-CoA required is determined.

    $2.19