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Cell BioChem

Discussing ATP Production

The major production of ATP during aerobic metabolism occurs when electrons from __________ and _____________ are transferred to _______________.

Metabolism

In the absence of oxygen, what is the primary purpose of fermentation?

Electron Transport Chain

1. One of the multiple questions was this: The electron transport chain is located predominantly in _________. The answer was: the inner membrane of the mitochondria. Why was this the answer?

Plotting RSA vs. Protein Percentage

Subcellular fractionation of rat liver resulted in the following samples: Fraction.......Speed (xg)......Volume (ml)......protein (mg/ml) P-1............2800..................10........................6 P-2............12000..................10......................4 P-3............27000..................8......

Metabolism in Biochemistry and reactions

2) The cell can transfer the high energy electrons from NADH into the mitochondria using either the glycerol phosphate shuttle or the malate-aspartate shuttle. Explain one of these two shuttles with a diagram and make sure to include details of total ATP yield. Show the structures or names of the molecules involved and also th

Determining length of DNA using known number of deoxyribonucleotide pairs

Give a) mathematical expression to calculate the number of possible sequences of nucleotide pairs with the usual base pairing, for a molecule of n-deoxyribonucleotide pairs. b) How would you determine the length of a DNA molecule in micrometers, with a known number of deoxyribonucleotide pairs?

Calculating pH, Ka, grams, and amounts of an acid for making a buffer

1. Calculate the pH of a solution which is 0.428 M in oxalic acid and 1.44 M in oxalate. 2. What is the pH of a solution that is 45.5 mM in acetic acid and 1.11 M in acetate? 3. A solution which is 3.19X10 to the -3 M in an acid (HA) and 6.78x10 to the -3 M in its conjugate base (A-) is found to have a pH of 8.65. What i

Determining the pKa of an acid.

For a weak electrolyte that ionizes as: HX --> H+ + X- construct a buffer with a ratio of 5 moles of HX/2.1 moles X-. The reaction is maintained at pH = 8.7. What is the pKa of the acid?

NaCl solution is discussed.

We did a lab with DNA standards. We made one to one solutions of onion DNA that we precipitated and a 4% NaCl soln. We measured the amount of DNA using spectrophotometers. When we boiled the solution before measurement, the NaCl precipitated. This made the solution cloudy and the absorbancy readings inaccurate. I'm wondering i