The major production of ATP during aerobic metabolism occurs when electrons from __________ and _____________ are transferred to _______________.
In the absence of oxygen, what is the primary purpose of fermentation?
1. One of the multiple questions was this: The electron transport chain is located predominantly in _________. The answer was: the inner membrane of the mitochondria. Why was this the answer?
If you isolate mitochondria and place them in buffer with a low pH, they begin to manufacture ATP. Why?
Plot the distribution profile for each enzyme as RSA (Relative Specific Activity) vs % protein.
Subcellular fractionation of rat liver resulted in the following samples: Fraction.......Speed (xg)......Volume (ml)......protein (mg/ml) P-1............2800..................10........................6 P-2............12000..................10......................4 P-3............27000..................8......
Please see the attached file.
State the steps involved in the generation of ATP by the mitochondrion.
2) The cell can transfer the high energy electrons from NADH into the mitochondria using either the glycerol phosphate shuttle or the malate-aspartate shuttle. Explain one of these two shuttles with a diagram and make sure to include details of total ATP yield. Show the structures or names of the molecules involved and also th
What is acetylcholine dehydrogenase?
The deoxyribonucleotide composition of phage T2 DNA is 2,00,000 pairs. What is the length of its DNA compliment in micrometers?
Give a) mathematical expression to calculate the number of possible sequences of nucleotide pairs with the usual base pairing, for a molecule of n-deoxyribonucleotide pairs. b) How would you determine the length of a DNA molecule in micrometers, with a known number of deoxyribonucleotide pairs?
1. Calculate the pH of a solution which is 0.428 M in oxalic acid and 1.44 M in oxalate. 2. What is the pH of a solution that is 45.5 mM in acetic acid and 1.11 M in acetate? 3. A solution which is 3.19X10 to the -3 M in an acid (HA) and 6.78x10 to the -3 M in its conjugate base (A-) is found to have a pH of 8.65. What i
To convert androsten-16-en-3-ol into androsterone in vitro, what specific series of changes in functional groups would have to be carried out?
If the hemoglobin tetramer had four identical non-interacting binding sites, what relative values would the intrinsic binding constants have?
What will happen to Glucose, Pyruvate and CO2 if cyanide stops the electron transport system?
A mutant strain of E.coli expresses trp operon mRNA but still cannot synthesize trypophan. How could this mutant phenotype result?
A mutant strain of E.coli has constitutive expression of the trp operon. How could this mutant phenotype result? Ideas are explained.
What would be the effect on lac operon expression in the following media that contains a strain of E.Coli? What if this mutant also contained an O^- mutation? a. Glucose b. Lactose c. Glucose + Lactose
By what processes are macromolecules transported in and out of a cell? Ideas are examined to illustrate.
What takes place at an ecological and physiological level during the freezing and thawing process of the wood frog?
For a weak electrolyte that ionizes as: HX --> H+ + X- construct a buffer with a ratio of 5 moles of HX/2.1 moles X-. The reaction is maintained at pH = 8.7. What is the pKa of the acid?
How do you calculate specific activity, total activity, and % radioactivity given the information in the attachment?
With particular emphasis on the biochemistry, discuss the machinery involved in the transcriptional process.
We did a lab with DNA standards. We made one to one solutions of onion DNA that we precipitated and a 4% NaCl soln. We measured the amount of DNA using spectrophotometers. When we boiled the solution before measurement, the NaCl precipitated. This made the solution cloudy and the absorbancy readings inaccurate. I'm wondering i
Double stranded DNA isolated from a bacteriophage has a molecular weight of 2.1x10E8 Da. (1) Using this information, how many amino acids would this DNA encode? (2) How many different proteins could be encoded by this DNA, if the protein molecular weight is 69 kDa? These ideas are depicted.
What are four similarities in the polymeric structure and synthesis of proteins and nucleic acids?