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Cell BioChem

Isomerization of a Phosphorylated Glucose Molecule

The isomerization of a phosphorylated glucose molecule from 1-p to 6-p form with standard free energy change=-1.74kcal/mole. Find the equilibrium concentration ratio of glucose-p in the two isometric states.

Picking the Best Inhibitor when Given Km, Kcat and Ki

Here are the following numbers I have calculated; We were given a substrate to plot againist velocity and 3 enzymes to plot as well. From the data: Enzyme A Ki= 2.575 | Kcat = 50 | Km= 3.33 Enzyme B Ki = 10.33 | Kcat = 16.65 | Km = 33.3 Enzyme C Ki= 1.00 | Kcat = 10 | Km = .2 I'm having tr

Artificial Cells

What are artificial cells? How are artificial cells produced? Examples of artificial cells and applications of artificial cell in biochemistry and medicine.

How Many Grams of Alcohol Will Be Produced?

Enzymes in yeast convert sugar (C6H12O6) to alcohol (C2H5OH) according to the following equation: C6H12O6 2 C2H5OH + 2 CO2 If you start with 270 grams of sugar, how many grams of alcohol will be produced?

Finding percentage of protein

Please see the attached file. Subcellular fractionation of rat liver resulted in the following samples: Fraction speed (xg) Volume(ml) Protein (mg/ml) P-1 2800 10 6 P-2 12000 10 4 P-3 27000 8 4 P-4 75000 5 2.5 S non-sedimentable 25 6.5 Anal

Terminal Electron Acceptor

The terminal electron acceptor during mitochondrial respiration is the electron transport in the inner mitochondrial membrane Explain.

Discussing ATP Production

The major production of ATP during aerobic metabolism occurs when electrons from __________ and _____________ are transferred to _______________.

Metabolism

In the absence of oxygen, what is the primary purpose of fermentation?

Electron Transport Chain

1. One of the multiple questions was this: The electron transport chain is located predominantly in _________. The answer was: the inner membrane of the mitochondria. Why was this the answer?

Question about Distribution profile

Plot the distribution profile for each enzyme as RSA (Relative Specific Activity) vs % protein. Subcellular fractionation of rat liver resulted in the following samples: Fraction speed (xg) Volume(ml) Protein (mg/ml) P-1 2800 10 6 P-2 12000 10 4 P-3 27000 8 4 P-4

Plotting RSA vs. Protein Percentage

Subcellular fractionation of rat liver resulted in the following samples: Fraction.......Speed (xg)......Volume (ml)......protein (mg/ml) P-1............2800..................10........................6 P-2............12000..................10......................4 P-3............27000..................8......

Finding relative specific activity of an enzyme

Subcellular fractionation of rat liver resulted in the following samples: Fraction Speed (xg) Volume (ml) protein (mg/ml) P-1 2800 10 6 Analysis of the fractions resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.

Metabolism in Biochemistry and reactions

2) The cell can transfer the high energy electrons from NADH into the mitochondria using either the glycerol phosphate shuttle or the malate-aspartate shuttle. Explain one of these two shuttles with a diagram and make sure to include details of total ATP yield. Show the structures or names of the molecules involved and also th

Calculate length of DNA in corn

A diploid (somatic) cell of corn has DNA of molecular weight 9 à? 10 12 [ten power twelve]. Determine a) the number of deoxyribonucleotide pairs, b) its length in micrometers and c) its length in inches.

Determining length of DNA using known number of deoxyribonucleotide pairs

Give a) mathematical expression to calculate the number of possible sequences of nucleotide pairs with the usual base pairing, for a molecule of n-deoxyribonucleotide pairs. b) How would you determine the length of a DNA molecule in micrometers, with a known number of deoxyribonucleotide pairs?

Calculating pH, Ka, grams, and amounts of an acid for making a buffer

1. Calculate the pH of a solution which is 0.428 M in oxalic acid and 1.44 M in oxalate. 2. What is the pH of a solution that is 45.5 mM in acetic acid and 1.11 M in acetate? 3. A solution which is 3.19X10 to the -3 M in an acid (HA) and 6.78x10 to the -3 M in its conjugate base (A-) is found to have a pH of 8.65. What i

Determining the pKa of an acid.

For a weak electrolyte that ionizes as: HX --> H+ + X- construct a buffer with a ratio of 5 moles of HX/2.1 moles X-. The reaction is maintained at pH = 8.7. What is the pKa of the acid?