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    Plotting RSA versus Protein Percentage

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    Subcellular fractionation of rat liver resulted in the following samples:

    Fraction.......Speed (xg)......Volume (ml)......protein (mg/ml)
    P-1............2800..................10........................6
    P-2............12000..................10......................4
    P-3............27000..................8........................4
    P-4............75000..................5........................2.5
    S............non-sedimentable..25....................6.5

    Analysis of the fractions resulted in the following enzyme activities expressed as
    nmoles/min/ml of sample fraction.

    Fraction......citrate synthase......catalase......phosphatase......aldolase
    P-1..................0.20........................0.10............0.80..................1.0
    P-2..................8.00........................1.00............9.00..................2.0
    P-3..................6.00........................7.00............14.0..................1.8
    P-4..................0.50........................17.5............12.0..................0.9
    S.....................0.04........................0.40............0.70..................15.0

    [RSA
    Relative Specific
    Activity ]..................P-1............ P-2............ P-3............ P-4............ S
    Citrate synthase...0.033..........2.00...........1.50............0.20........ 0.006
    Catalase...............0.016..........0.25...........1.75............7.00.........0.061
    Phosphatase........0.13............2.25............3.50............4.80........0.107
    Aldolase...............0.16............0.50............0.45............0.36.........2.30

    Plot the distribution profile for each enzyme as RSA vs. % protein

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    https://brainmass.com/biology/cell-biochem/plotting-rsa-versus-protein-percentage-11541

    Solution Preview

    So you want to plot a distribution profile for each enzyme as RSA vs. protein percentage.

    First calculate the percentage of proteins in the given samples. Since the ...

    Solution Summary

    The answer is given along with an attachment of a diagram to generalize how to plot the graphs.

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