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Plotting RSA versus Protein Percentage

Subcellular fractionation of rat liver resulted in the following samples:

Fraction.......Speed (xg)......Volume (ml)......protein (mg/ml)
P-1............2800..................10........................6
P-2............12000..................10......................4
P-3............27000..................8........................4
P-4............75000..................5........................2.5
S............non-sedimentable..25....................6.5

Analysis of the fractions resulted in the following enzyme activities expressed as
nmoles/min/ml of sample fraction.

Fraction......citrate synthase......catalase......phosphatase......aldolase
P-1..................0.20........................0.10............0.80..................1.0
P-2..................8.00........................1.00............9.00..................2.0
P-3..................6.00........................7.00............14.0..................1.8
P-4..................0.50........................17.5............12.0..................0.9
S.....................0.04........................0.40............0.70..................15.0

[RSA
Relative Specific
Activity ]..................P-1............ P-2............ P-3............ P-4............ S
Citrate synthase...0.033..........2.00...........1.50............0.20........ 0.006
Catalase...............0.016..........0.25...........1.75............7.00.........0.061
Phosphatase........0.13............2.25............3.50............4.80........0.107
Aldolase...............0.16............0.50............0.45............0.36.........2.30

Plot the distribution profile for each enzyme as RSA vs. % protein

Solution Preview

So you want to plot a distribution profile for each enzyme as RSA vs. protein percentage.

First calculate the percentage of proteins in the given samples. Since the ...

Solution Summary

The answer is given along with an attachment of a diagram to generalize how to plot the graphs.

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