Purchase Solution

Question about Distribution profile

Not what you're looking for?

Ask Custom Question

Plot the distribution profile for each enzyme as RSA (Relative Specific Activity) vs % protein.

Subcellular fractionation of rat liver resulted in the following samples:

Fraction speed (xg) Volume(ml) Protein (mg/ml)

P-1 2800 10 6

P-2 12000 10 4

P-3 27000 8 4

P-4 75000 5 2.5

S non-sedimentable 25 6.5

Analysis of the frations resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.

Fraction Citrate synthase
P-1 0.20
P-2 8.00
P-3 6.00
P-4 0.50
S 0.04

Plot the distribution profile for each enzyme as RSA (Relative Specific Activity) vs % protein.

I need the graph in any format available (excel, word...etc) Please don't send me info on how to do the graph. I just need it done because I'm working all night tonite and have no time. Thank you for your help.

Purchase this Solution

Solution Summary

Solution contains the % Protein for each fraction, the enzyme activity in nmoles/min/ml and a graph plotted with the % protein (independent variable x) versus the enzyme activity in nmoles/min/ml (dependent variable y).

Solution Preview

See attachment for full solutions.

Here are the % Protein for each fraction

Fraction P-1 % = (60/307) x 100 % = 19.5%
Fraction P-2 % = (40/307) x ...

Purchase this Solution


Free BrainMass Quizzes
Pregnancy Knowledge

How much do you know about being pregnant? Test your Pregnancy IQ with this quiz!

Understanding the Musculoskeletal system

Introduce and understand basic information how the skeletal system and muscular system work in close concert with one another. And how their interaction between muscle and bone, as they work together to allow us movement.

Birth 101

Do you know about childbirth? Find out with this quiz.

Comfort Measures For Labor

Are you ready to doula someone through labor?

Vision and Oculomotor Control

This quiz will test the student's knowledge of the neural underpinnings of the visual system and its central pathways.