Subcellular fractionation of rat liver resulted in the following samples:
Fraction speed (xg) Volume(ml) Protein (mg/ml)
P-1 2800 10 6
P-2 12000 10 4
P-3 27000 8 4
P-4 75000 5 2.5
S non-sedimentable 25 6.5
Analysis of the frations resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.
Fraction Citrate synthase
Plot the distribution profile for each enzyme as RSA (Relative Specific Activity) vs % protein.
I need the graph in any format available (excel, word...etc) Please don't send me info on how to do the graph. I just need it done because I'm working all night tonite and have no time. Thank you for your help.
See attachment for full solutions.
Here are the % Protein for each fraction
Fraction P-1 % = (60/307) x 100 % = 19.5%
Fraction P-2 % = (40/307) x ...
Solution contains the % Protein for each fraction, the enzyme activity in nmoles/min/ml and a graph plotted with the % protein (independent variable x) versus the enzyme activity in nmoles/min/ml (dependent variable y).