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    Question about Distribution profile

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    Plot the distribution profile for each enzyme as RSA (Relative Specific Activity) vs % protein.

    Subcellular fractionation of rat liver resulted in the following samples:

    Fraction speed (xg) Volume(ml) Protein (mg/ml)

    P-1 2800 10 6

    P-2 12000 10 4

    P-3 27000 8 4

    P-4 75000 5 2.5

    S non-sedimentable 25 6.5

    Analysis of the frations resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.

    Fraction Citrate synthase
    P-1 0.20
    P-2 8.00
    P-3 6.00
    P-4 0.50
    S 0.04

    Plot the distribution profile for each enzyme as RSA (Relative Specific Activity) vs % protein.

    I need the graph in any format available (excel, word...etc) Please don't send me info on how to do the graph. I just need it done because I'm working all night tonite and have no time. Thank you for your help.

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    https://brainmass.com/biology/cell-biochem/question-about-distribution-profile-11976

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    Here are the % Protein for each fraction

    Fraction P-1 % = (60/307) x 100 % = 19.5%
    Fraction P-2 % = (40/307) x ...

    Solution Summary

    Solution contains the % Protein for each fraction, the enzyme activity in nmoles/min/ml and a graph plotted with the % protein (independent variable x) versus the enzyme activity in nmoles/min/ml (dependent variable y).

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