Finding relative specific activity of an enzyme
Subcellular fractionation of rat liver resulted in the following samples:
Fraction Speed (xg) Volume (ml) protein (mg/ml)
P-1 2800 10 6
Analysis of the fractions resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.
Fraction citrate synthase catalase phosphatase aldolase
P-1 0.20 0.10 0.80 1.0
Question #1= From the above results determine the relative specific activity (RSA) of each enzyme.
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The relative specific activity of an enzyme is calculated. The relative specific activity of an enzyme is analyzed.
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