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Finding relative specific activity of an enzyme

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Subcellular fractionation of rat liver resulted in the following samples:

Fraction Speed (xg) Volume (ml) protein (mg/ml)

P-1 2800 10 6

Analysis of the fractions resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.

Fraction citrate synthase catalase phosphatase aldolase

P-1 0.20 0.10 0.80 1.0

Question #1= From the above results determine the relative specific activity (RSA) of each enzyme.

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Solution Summary

The relative specific activity of an enzyme is calculated. The relative specific activity of an enzyme is analyzed.

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