Determining length of DNA using known number of deoxyribonucleotide pairs
Give a) mathematical expression to calculate the number of possible sequences of nucleotide pairs with the usual base pairing, for a molecule of n-deoxyribonucleotide pairs.
b) How would you determine the length of a DNA molecule in micrometers, with a known number of deoxyribonucleotide pairs?
If cytosine makes up 22% of the nucleotides in a sample of DNA from an organism, then adenine would make up what % of the bases.
A) 22 B) 44 C) 28 D) 56
Suppose 25,000 μm is 1 inch. Enumerate a formula to find the length of a DNA molecule in inches
a) if the length is known in μm and
b) only the number of nucleotide pairs is given.
A double stranded DNA virus has 200,000 base pairs. Determine the number of nucleotides present and the total number of complete spirals. How long will this DNA be?
Based on the attached data collected from DNA isolation from two different bacterial cultures:
1. Complete the table by determining the percentage content of the corresponding complementary bases for each bacterial population. On what assumptions are your calculations made?
2. Describe the relationship between Tm and base
Two duplex (ie. double stranded) DNA fragments are shown below. Under identical conditions (ie. ionic strength, buffer composition, temperature, etc.), which double-stranded fragment would denature at the lower temperature?
Fragment A:
5' A-A-G-T-A-G-T-T-T-C-A 3'
3' T-T-C-A-T-C-A-A-A-G-T 5'
Fragment B:
5' G-C-A-G-G-T-C-G-C-
An Escherichia coli plasmid in a closed circle with 5, 200 bp and naturally has 26 negative super coil turns.
1.Calculate the linking number for this plasmid. Assume it is adopting 10.4 bps/turn of the regular B-DNA.
2.The above super coiled plasmid has been linearized by treating with a dsDNA breaking enzyme. What's the len
1. Bacteriophage M13 infects E. coli differently from the way bacteriophage T2 does. The M13 coat is removed in the inner membrane of the bacterial cell, where it is sequestered during phage replication. It is subsequently used to package the newly replicated phage DNA to create progeny phage. Why would this make M13 less su
