1. Biology
2. Biological Chemistry
3. Cell BioChem
4. 13007

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Subcellular fractionation of rat liver resulted in the following samples:

Fraction speed (xg) Volume(ml) Protein (mg/ml)

P-1 2800 10 6

P-2 12000 10 4

P-3 27000 8 4

P-4 75000 5 2.5

S non-sedimentable 25 6.5

Analysis of the frations resulted in the following enzyme activities expressed as nmoles/min/ml of sample fraction.

Fraction Citrate synthase
P-1 0.20
P-2 8.00
P-3 6.00
P-4 0.50
S 0.04

Question: Find the % protein for each fraction.

Attachments

• % Protein.doc