11. Define restriction enzymes, and outline how they are used to make recombinant DNA. 12. Outline the PCR process and provide an example of its use. 13. Describe five ways of getting DNA into a cell. 14. Describe how a gene library is made. 15. Differentiate between cDNA and synthetic DNA. 16.
1. Describe the use of a DNA probe and PCR for: 1. Rapid identification of an unknown bacterium. 2. Determining which of a group of bacterium are most closely related. 2. After contact with a patient's spinal fluid, a lab technician developed fever, nausea, and purple lesions on her neck and extremities. A throat culture
When phage λ is grown on a Tn10-containing E. coli host, rare phage that transduce tetracycline-resistance can be isolated. You isolate DNA from λ and from λ-tet and cut each with EcoRI and BamHI and see the following sized fragments on an agarose gel: Tn10 is 9kb long, with one EcoRI site 3.5kb from its left end (and the
1.Herschley-Bulkley Model Please describe in detail this model indicating the meaning of each parameter and their range. Please list and describe alternative THREE PARAMETER Models. 2.Creep Test Please describe in detail this test and explain why is so useful in characterizing viscoelasticity. 3.Relaxation test
Discuss why prokaryotes tend to be small relative to eukaryotic cells. Discuss why size may be limited in cells of eukaryotic organisms bases on their function. Provide examples and incorporate resources as necessary.
8. The virulence of an organism may be enhanced by all the following except a. Its ability to produce exotoxins. b. Its ability to overcome body defense. c. Its ability to spread through the tissues. d. Its ability to grow on artificial laboratory media. 15. A scientist who studies the pattern of disease in a commun
Please review the 5 questions for correctness. There were a couple that I was unable to answer. 5. Cyst formation by certain protozoa permits a. Survival in the arthropod vector. b. Enhanced reproduction by the protozoan. c. Passage through the stomach acid. d. Using carbon dioxide as an energy source. 16. An amoeb
Please confirm correctness of the attached 8 questions. 1. Yeasts produce mainly by a. Conidia b. Budding c. Sporangia d. Basidia 13. The retrovirus that leads to AIDS a. Has no enveople b. Contains RNA in its genome. c. Uses reverse transcriptase to convert DNA to RNA. d. Multiplies only within the brain cell
You are a graduate student working on a followship for a biotech companny .You are part of a group that will perform some scientiific research. You recieve various soil samples shipped to you from remote areas of the world. You and your team members need to evaluate these samples
A nutritionally defective E.coli strain grows only on meduim containing thymine, whereas another nutritionally defective strain grows only on meduim containing leucine. When these two strains are grown together , a few progeny are able to grow on minimal meduim with neither leucine nor thymine. How can this result be explained?
Protist is much more complex then cells of multiceller organisms. Does this mean that the Protist is engaged in more complex activities then multicellular organism and if not why should the Protistan cell be much more complicated?
1. Select a molecule. List the atoms that that molecule is composed of and describe the type of bond that holds those atoms together. Be sure to explain how this bond works. 2. Often, prokaryotic cells exist as simple unicellular organisms, but in some species, prokaryotic cells can grow together in colonies or filaments. In
What distinguishes a eukaryotic cell from a prokaryotic cell? Why embryos are an excellent source for stem cells? Why do cows have the ability to breakdown cellulose into glucose and humans can not digest cellulose?
We are suppose to explain what type of mutation could cause for color variation in certain colonies of E. coli. We first started off with one normal strand of the E. coli bacteria, and after UV light exposure we need to propose a theory of what typeof mutation could cause a single colony to mutate into 5 possible colors (white
Can microbiology assist human genetics? How the alkaptonuria (black urine disease) mutation was identified? Many of the processes that convert one material to another are common to many organisms. Some were preserved during evolution and they are similar among bacteria, plants, animals and humans.
Give the name for the type of cells that are simple in structure and are usually surrounded by a thick cell wall for protection and shape.
What phylum are the "Sun Animalicules" in? a. Heliozoa b. Actinopoda c. Acanthocephala d. Amoeba Radiolarians are most kin to the: a. heliozoa b. amebas c. paramecia d. rotifers Which type of diatom can move the best? a. centrates b. pennates c. helical d. rhombus What are food vacuoles used for? a. captu
What is Mycology? A. The study of molds B. The study of algae C. The study of Mutualism D. The study of fungi What are the 5 Kingdoms of living organisms? A. plants, animals, viruses, insects, bacteria B. animals, plants, bacteria, viruses, humans C. plants, animals, fungi, protista, strameopila D. bacteria, animals.
___ rust and smut fungi ___ Agaricus brunnescens ___ Armillaria ostoyae ___ Amanita muscaria ___ Amanita phalloides ___ truffles and morels ___ yeasts ___ Candida albicans ___ Penicillium ___ Aspergillis ___ Neurospora crassa A. "Flavor" Camembert and Roquefort cheeses; produc
_____saprobes _____parasites _____mycelium _____fungi _____hypha _____ zygomycetes, sac fungus, club fungi _____spores _____extracellular digestion and absorption A. Nonmotile reproductive cells and multicelled structures, often walled; germinate following dispersal from the parent body. B. Represent major lineages of
1. Term that describes the pink-colored E. coli. on this Gram-stained slide. a. Gram-negative b. Gram-positive 2. The round shaped bacterium is called _____. a. coccus b. spirillum c. bacillus 3. The oblong shaped bacterium is called ____. a. coccus b. spirillum c. bacillus 4. The wavy shaped bacterium is called
What term refers to a cell that has no nucleus or membrane bound organelles, such as bacteria? a. prokaryotic b. eukaryotic c. planktonic d. pathogenic What term refers to a cell that has a nucleus and membrane bound organelles, such as plant and animal cells? a. prokaryotic b. eukaryotic c. planktonic d. pathogenic
Bacteria never form any sort of grouping that resembles a multicelled organism with an internal division of labor among its cells. Think about the attributes of prokaryotic bacterial cells and the eukaryotic cells of which all multicellular organisms are comprised. Develop some hypotheses explaining why only eukaryotic cells hav
I am conducting a yearlong research project where I am testing a new medication, telithromycin, against 4 different types of bacteria. I will then compare its activity to that of another medication, levofloxacin, that is already known to work against these 4 bacteria. I have measured zones of inhibition for each medication vs. e
#6 Macrophages perform the final job of removing tissue debris and other products of infection. Indicate some of the possible effect when these scavengers cannot successfully complete the work of phagocytosis. (1pt.) #11 A patient with chronic diarrhea was found to lack IgA in his secretions, although he had a normal leve
Question: Activities of the following enzymes - protease, amylase, and lipase. I want detail on the activities of these enzymes in relation to food microbiology. I am not too sure how to approach this question.
Briefly describe the methods used by bacteria and fungi to tolerate environmental heavy metals. Include references and your views on the potential commercial applications of bacteria and fungi.