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Microbiology Questions

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Please confirm correctness of the attached 8 questions.

1. Yeasts produce mainly by
a. Conidia
b. Budding
c. Sporangia
d. Basidia

13. The retrovirus that leads to AIDS
a. Has no enveople
b. Contains RNA in its genome.
c. Uses reverse transcriptase to convert DNA to RNA.
d. Multiplies only within the brain cells of the body.

14. Which one of the following symptoms is not associated with HIV infection?
a. Periods of sweating during sleeping hours.
b. Low-grade fever that remains for weeks or months at a time.
c. Long lasting diarrhea.
d. Painful ulcers in the gastrointestinal tract.

21. Influenza like prodrome, blood hemorrhaging and acute respiratory failure are associated with which one of the following viruses?
a. Cytomegalovirus.
b. Hantavirus.
c. HIV
d. Rabies virus.

23. Yeasts are plentiful
a. Where there are orchards or fruits.
b. In meat packing facilities.
c. In human blood.
d. Where dogs, cats, and other household pets are kept.

37. Dimorphic fungi are those that display different morphological properties at different temperatures?
a. True
b. False

39. Cases of infectious mononucleosis are accompanied by sore throat, mild fever, and a high count of the white blood cells called lymphocytes.
a. True - specifically B-lymphocytes
b. False

49. Conidia are sexually produced spores used for reproduction by such fungi as penicillium.
a. True
b. False - Conidia is asexual

See attached file for full problem description.

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Microbiology Questions- Direct and Viable Counts

The depth of the hemocytometer sample box is 0.1mm for all questions.

Please show all calculations.

1. Why might the direct and variable counts of a suspension disagree?
2. You dilute an original sample 1:30. You then count the number of yeast in the 1:30 dilution. You count an average of 7 cells in the 1/25 mm^2 sized boxes. What is the density, in cells/ml, of the original sample?
3. You count the number of bacteria in 5 of the 1/400 mm^2 boxes of the central grid on the hemocytometer. Your results are: 12 cells in box #1, 17 cells in box #2, 17 cells in box #3, 14 cells in box #4 and 16 cells in box #5. You are counting a 1:10 dilution of the original sample. What is the density of the original culture (in cells/ml)?

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