Explore BrainMass
Share

Conducting Microbiology Calculations

This content was STOLEN from BrainMass.com - View the original, and get the already-completed solution here!

Please help with the following problem Show all calculations.

The depth of the hemocytometer sample box is 0.1mm for all questions.

1. You count 74 cfu on a pour plate. The plate was prepared by spreading 0.2 ml of a 1:10,000 dilution of the original sample. What is the density of the original culture (in cells/ml)?

2. You have a culture of yeast that is at a density of 4.76 x 10^6 cells/ml. You dilute the sample 1:100, and then again 1:100, and finally you dilute the sample an additional 1:3. You add 0.1 ml of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony forming units do you expect to count on your plate the next day?

3. What is the average size of a yeast cell?

© BrainMass Inc. brainmass.com October 25, 2018, 7:28 am ad1c9bdddf
https://brainmass.com/biology/prokaryotic-cells/conducting-microbiology-calculations-504603

Solution Preview

1. CFU is the abbreviation for colony forming unit. In other words, in 0.2 ml of the dilution, there are 74 ...

Solution Summary

The following posting helps with problems that involve conducting microbiology calculations. Step by step calculations are shown for each.

$2.19
See Also This Related BrainMass Solution

International Trade

You are eating a fruit salad that contains the following ingredients: bib lettuce, bananas, apples, coconut, kiwi, mango, avocado, figs, and almonds. Make a list or each item in your salad and their countries of origin.

Include why each fruit grows well in that particular area.

Then, determine crops, in addition to the fruit salad items, are grown in your area.

Address the following topics:

How many items in your salad were locally grown?

How dependent on international trade and trade between states are we?

Discuss the impact of the movement of food around the world.

View Full Posting Details