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Recombinant DNA

Recombinant DNA is the merging of DNA sequences from a variety of species to add, remove, enhance, and modify the expression of traits. The genetic code is called ‘universal’ because it is used by all living organisms, the distinction of species come from the different expressions of the code by varying DNA sequences. The universality factor of genes allows sequences from multiple organisms to be pieced together and inserted into a plasmid or vector. The function of recombinant DNA is to be expressed, thus producing new codons and eventually proteins in a specific organism.

There are two stages of recombinant DNA production which is first producing the recombinant DNA and then replicating it. In replication of the recombinant data there are two methods: molecular cloning and polymerase chain reaction (PCR). The difference is that molecular cloning is in vivo, within a cell whilst PCR is in vitro, within a test tube.  In producing the recombinant data, sequences of target DNA of a species is removed with restriction enzymes and then ‘sticky ends’ are added by DNA ligase. Hybridization occurs when DNA ligase inserts the foreign sequence into a plasmid, joining the sticky ends to plasmid sequence.

The recombinant DNA can then be cloned by either molecular cloning or PCR. In molecular cloning, the recombinant plasmid is inserted into a cell which then DNA replication expresses and enhances the traits of the foreign DNA. In polymerase chain reaction (PCR), the recombinant DNA is placed in a test tube with enzymes and it undergoes cycles of heating and cooling, where it is amplified to produce thousands of copies by DNA polymerase

DNA Transformation

In this experiment, we will change the genotype and therefore the phenotype of a bacterium by genetically transforming an organism by introducing foreign DNA into its genome. This is called transformation. In this exercise, we will use the bacterial plasmid as a source of transforming DNA. The plasmid we are using is calle

Research on Gene Splicing

I need to provide a response to the following question. Could you please assist? Provide research on gene splicing, a DNA technology that bio-engineers can use to create organisms with traits never before seen in nature (herbicide-resistant crops, mice with fluorescent organs, yeast cells that smell like wintergreen, etc.).

Exploring Gene Expression, Population Evolution and DNA Technology

Question 1 - In which we explore gene expression. A. Explain how differential gene expression yields a variety of cell types in a multi-cellular eukaryote. B. Explain how DNA is packaged into chromosomes. Explain how packing influences gene expression. C. Explain the process and significance of alternative DNA splicing.


What is recombinant DNA? How do we cut and paste DNA