Polylinker Site in Plasmid or Bacteriophage Cloning Vector

Related BrainMass Solutions

• Vectors

  75894 Vectors Briefly describe the purpose of the following features found in all or many plasmid cloning vectors.

• Engineering a recombinant plasmid DNA to over-express protein

  to amplify the cDNA and allow cloning into the pGEX-4T-2 vector. as shown on the map of the pGEX vector, we can use BamHI,EcoRI,SmaI,SalI,XhoI or NotI for cloning. 4-show the cDNA sequence and corresponding primer sequence for both the upstream and

• Using bioinformatics and DNA sequence analysis

  have recognition sequences specified by six nucleotides) are good for day-to-day cloning work: You are using 6-cutters in the experiments you are performing in the lab, because they cut frequently enough that there are one or two sites in the plasmid

• DNA Technology

  Describe DNA sequencing with reference to the cloning of a DNA fragment in a bacteriophage M13 based cloning vector. 2. Describe the use of a expression vector to isolate the gene from a cDNA library that codes for a specific protein. 1.

• Intrepreting results of control studies in a cloning study.

  "There are two primary uses for alkaline phosphatase in DNA manipulations: ? Removing 5' phosphates from plasmid and bacteriophage vectors that have been cut with a restriction enzyme.

• Examining DNA plasmid restriction digest

  How do you know what size your recombinant plasmid is after cloning and ligation of the insert into the plasmid. 2.

• Agarose gel electrophoresis - why does my bp not add up?

  EcoRI cuts pUC18 only once in the multiple cloning site (according to the map I am looking at, the digesting with EcoRI will not break up your plasmid.

• Lambda Phage

  How has it been used in biotechnology? Explain how it is used as a vector for the cloning of recombinant DNA. Why is it important to know about restriction enzyme sites in phage lambda DNA?

• Recombinant DNA Technology Discussion

  Now, that the cDNA is pasted into a plasmid or a virus DNA, it is incubated in the presence of the bacteria (from which the plasmids originally came) or the virus. (A plasmid is a small circular extra-chromosomal piece of DNA found in bacteria.)