Using bioinformatics and DNA sequence analysis
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Why are we most interested in using 6 base pair cutters?
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Solution Summary
The expert uses bioinformatics and DNA sequence analysis to determine the restriction endonuclease site.
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Restriction enzymes are classified as endonucleases. Their biochemical activity is the hydrolysis ("digestion") of the phosphodiester backbone at specific sites in a DNA sequence. By "specific" we mean that an enzyme will only digest a DNA molecule after locating a particular sequence
Enzymes with recognition sequences from 4 to 8 nucleotides in length each have uses in genetic engineering.
6-cutters
(i.e. enzymes that have recognition sequences specified by six nucleotides) are good for day-to-day cloning work: You are using 6-cutters in the ...
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