Why are we most interested in using 6 base pair cutters?© BrainMass Inc. brainmass.com December 19, 2018, 7:51 pm ad1c9bdddf
Restriction enzymes are classified as endonucleases. Their biochemical activity is the hydrolysis ("digestion") of the phosphodiester backbone at specific sites in a DNA sequence. By "specific" we mean that an enzyme will only digest a DNA molecule after locating a particular sequence
Enzymes with recognition sequences from 4 to 8 nucleotides in length each have uses in genetic engineering.
(i.e. enzymes that have recognition sequences specified by six nucleotides) are good for day-to-day cloning work: You are using 6-cutters in the ...
The expert uses bioinformatics and DNA sequence analysis to determine the restriction endonuclease site.