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Vectors

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Briefly describe the purpose of the following features found in all or many plasmid cloning vectors.

a) origin of replication (ori)
b) multiple cloning site (polylinker)
c) antibiotic resistane gene

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Hi

There are lots of good sites describing plasmid cloning vectors (which I used to get this information):

http://www.web-books.com/MoBio/Free/Ch9A4.htm
http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm
http://dwb.unl.edu/Teacher/NSF/C08/C08Links/mbclserver.rutgers.edu/~sofer/cloningvectors.html

A cloning vector is used as a carrier for the insertion and expression of a gene of interest, and as ...

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