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Vectors

Briefly describe the purpose of the following features found in all or many plasmid cloning vectors.

a) origin of replication (ori)
b) multiple cloning site (polylinker)
c) antibiotic resistane gene

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Hi

There are lots of good sites describing plasmid cloning vectors (which I used to get this information):

http://www.web-books.com/MoBio/Free/Ch9A4.htm
http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm
http://dwb.unl.edu/Teacher/NSF/C08/C08Links/mbclserver.rutgers.edu/~sofer/cloningvectors.html

A cloning vector is used as a carrier for the insertion and expression of a gene of interest, and as ...

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